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A1WGU0 (SYL_VEREI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Veis_1071
OrganismVerminephrobacter eiseniae (strain EF01-2) [Complete proteome] [HAMAP]
Taxonomic identifier391735 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeVerminephrobacter

Protein attributes

Sequence length907 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 907907Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009460

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif651 – 6555"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6541ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A1WGU0 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 2515C1206D9A9CB4

FASTA907101,177
        10         20         30         40         50         60 
MQDKYSPQDI ERAAQDDWRA RDAYRVTQDA GKKKFYACSM LPYPSGKLHM GHVRNYTIND 

        70         80         90        100        110        120 
MLTRYLRMNG YNVLMPMGWD AFGLPAENAA IKNGMPPARW TRENIAYMKQ QMQALGLAID 

       130        140        150        160        170        180 
WSRELATCDP GYYKWNQWLF LKMLDKGIAY RKTQVVNWDP VDQTVLANEQ VNEGKGWRTG 

       190        200        210        220        230        240 
AVVEKREIPG YYLKITDYAE ELLAFVTDDR LPGWPERVKL MQENWIGKSE GLRFAFPHDV 

       250        260        270        280        290        300 
RGDDGALIGG GRLYVFTTRA DTIMGVTFCA VAPEHPLASH AAKSNPALAA FIDECRSGGT 

       310        320        330        340        350        360 
TEAALATQEK KGLRTGLYVT HPLTDEPLEV WVGNYVLMGY GDGAVMGVPA HDERDFAFAL 

       370        380        390        400        410        420 
KYGIEIRQVV LVDGEHFDYR RWQDWYGDKQ RGVTINSDSF SGLHHKEAVQ AVAHVLEQKG 

       430        440        450        460        470        480 
LGEKKTTWRL RDWGVSRQRY WGTPIPIIHC DQHGAVPVPE KDLPVVLPQD CIPDGTGNPL 

       490        500        510        520        530        540 
HSHQGFHAGV TCPVCGKSAR RETDTMDTFV DSAWYFMRYC DPKNDQAMVA EGTDYWMRDP 

       550        560        570        580        590        600 
QQATGGSGMD QYIGGIEHAI LHLLYARFWT KVMRDLGLVK VDEPFNRLLT QGMVLNHIYS 

       610        620        630        640        650        660 
RRNDNGGREY FWPQDVEQVH DAAGKIIGAR LIRAVGDWPA GSAIDYEGMG TMSKSRNNGV 

       670        680        690        700        710        720 
DPQELIGKYG ADTARLHTMF TAPPEAALEW NDAAVEGSHR FLRRVWNFGL QLHAAGMTAA 

       730        740        750        760        770        780 
SASLAGAGAP QTRNPPAFGK PAKALRREIH QLLRQVDYDY QRMQYNTVVS GAMKMLNALE 

       790        800        810        820        830        840 
NFKASDPASQ ASDPLALTEG FGILLRCLYP VTPHIAHSLW RDLGYAAAVG ELLDAPWPQV 

       850        860        870        880        890        900 
DGQALVQDEI ELMLQINGKL RGSILVPAQA SQAAIERIAR ASPAALAAGA VPKRVIVVPG 


RLVNLVF 

« Hide

References

[1]"Complete sequence of chromosome 1 of Verminephrobacter eiseniae EF01-2."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: EF01-2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000542 Genomic DNA. Translation: ABM56847.1.
RefSeqYP_995865.1. NC_008786.1.

3D structure databases

ProteinModelPortalA1WGU0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING391735.Veis_1071.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM56847; ABM56847; Veis_1071.
GeneID4694883.
KEGGvei:Veis_1071.
PATRIC24015599. VBIVerEis120356_1195.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycVEIS391735:GHY5-1082-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR024909. Cys-tRNA/MSH_ligase.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_VEREI
AccessionPrimary (citable) accession number: A1WGU0
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: April 16, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries