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A1WCP8 (SYL_ACISJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Ajs_3917
OrganismAcidovorax sp. (strain JS42) [Complete proteome] [HAMAP]
Taxonomic identifier232721 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeAcidovorax

Protein attributes

Sequence length910 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 910910Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009285

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif658 – 6625"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6611ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A1WCP8 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: C0549663194C215C

FASTA910101,205
        10         20         30         40         50         60 
MQDKYNHTEV ERAAHAHWNA NDAYRVTEDQ AKPKFYACSM LPYPSGKLHM GHVRNYTIND 

        70         80         90        100        110        120 
MLTRSLRMKG HNVLMPMGWD AFGLPAENAA LKNGVPPAQW TYDNIAYMKK QMQAMGLAID 

       130        140        150        160        170        180 
WSREIATCDP DYYKWNQWLF LKMLDKGIAY RKTQVVNWDP VDQTVLANEQ VIDGRGWRTG 

       190        200        210        220        230        240 
ALVEKREIPG YYLKITDYAQ ELLDHVQIGN EKATLTGWPD KVRLMQENWI GKSAGVRFAF 

       250        260        270        280        290        300 
PHDIRNAAGE RIQDGKLYVF TTRADTIMGV TFCAVAPEHP LAQHAAASNA ALAAFIEECK 

       310        320        330        340        350        360 
KGGTTEAELA LKEKEGMPTG LFVTHPLTGE QVEVWVGNYV LMSYGDGAVM GVPAHDERDF 

       370        380        390        400        410        420 
AFALKYQLPI KQVVLVDGET FDFHQWQDWY GDKERGVTIN SGNFSGLSYQ DAVAAVAHAL 

       430        440        450        460        470        480 
AEKGLGELKT TWRLRDWGIS RQRYWGTPIP IIHCESCGAV PVPEKDLPVV LPQDLVPDGS 

       490        500        510        520        530        540 
GNPLAKCEAF LKVDCPCCGK PARRETDTMD TFVDSSWYFM RYCDPKNRDA MVAGGTDYWM 

       550        560        570        580        590        600 
RDQKAATGGS GMDQYIGGIE HAILHLLYAR FWTKVMRDLG LVKVDEPFTK LLTQGMVLNH 

       610        620        630        640        650        660 
IYSRRTAKGA KDYFWPHDVE HVYDEAGKIV GAKLKNPAES GDGLLPVGTP IDYEGVGTMS 

       670        680        690        700        710        720 
KSKNNGVDPQ QLIEKYGADT ARLYTMFTAP PELTLEWNDA AVEGSYRFLR RVWNFGVKLS 

       730        740        750        760        770        780 
AIDKDAALAS VAGAASLKDV QFGKEAKALR LEIHTVLKQV DYDYQRMQYN TVVSGAMKMI 

       790        800        810        820        830        840 
NALEDFKATD SAGAQVALIE GFGILLRVLY PATPHIAHVL WDELGYAGTL GDLLDAAWPQ 

       850        860        870        880        890        900 
VAPDALVQDE LELMLQVNGK LRGAIRVAAS ADKAAIEQAA LASEDFHKFA EGKAPKKVII 

       910 
VPGRLVNVVV 

« Hide

References

[1]"Complete sequence of chromosome 1 of Acidovorax sp. JS42."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JS42.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000539 Genomic DNA. Translation: ABM44023.1.
RefSeqYP_988099.1. NC_008782.1.

3D structure databases

ProteinModelPortalA1WCP8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING232721.Ajs_3917.

Proteomic databases

PRIDEA1WCP8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM44023; ABM44023; Ajs_3917.
GeneID4673423.
KEGGajs:Ajs_3917.
PATRIC20693275. VBIAciSp27161_4138.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAMAFAGPP.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycASP232721:GHWE-3980-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_ACISJ
AccessionPrimary (citable) accession number: A1WCP8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: May 14, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries