Reviewed, UniProtKB/Swiss-Prot A1WCB1 (ACEK_ACISJ)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	Ajs_3777

Organism

Acidovorax sp. (strain JS42) [Complete proteome] [HAMAP]

Taxonomic identifier

232721 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	609 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity.
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 609	609	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000288278
Regions
	Nucleotide binding	325 – 331	7	ATP By similarity
Sites
	Active site	381	1	By similarity
	Binding site	346	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1WCB1-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 6C511FB2FF3DB4CB	FASTA	609	71,044
	10 20 30 40 50 60 MFPERLDAPQ AYDIAKAMMD GFNRHYRLFR AESARAKHRF ETADWHGQQR AQRERIEFYD 70 80 90 100 110 120 LRVRECVRRL DKEFNAGALP MDVWHQIKLH YIGLMVNHLQ PELAETFFNS VTTKILHRTH 130 140 150 160 170 180 FHNDFIFVRP AVSTEYIESD DPGARPTYRA YYPSRDNLHE TVVRIVEHCA LQRDFENLPR 190 200 210 220 230 240 DAGHVVQALQ QRLGAVKLRT NFQVQVLSSL FFRNKGAYLV GKVINGYNEL PFALPILHGE 250 260 270 280 290 300 DGRLLIDAVL FGENDLQMLF SFARAYFMVD MEIPSAYVQF LRSLMPRKPR AELYTALGLA 310 320 330 340 350 360 KQGKTLFYRD FLHHLRYSTD KFRIAPGIKG MVMLVFDLPS FPYVFKLIKD QFPAPKDTTR 370 380 390 400 410 420 EQVQGKYLLV KQHDRVGRMA DTLEYSLVAF PRERFSDELI EEIRRHAPSQ IEISDRDGDG 430 440 450 460 470 480 RQEVIIAHLY IERRMIPLNI HLQECFDTGL DKPEARSALE HAVTEYGNAI KDMVAANIFP 490 500 510 520 530 540 GDMLWKNFGI TRNGKVVFYD YDEIEYLTDC NFRRVPPPRC EEDEVSGEVW WPVGPHDVFP 550 560 570 580 590 600 ETFGPFLLGN DSVREAFMRH HADLLDVEFW QSHKERIQAG HLYDVFPYDS ARRFRCASPS SFQTQGDST

References

[1]

"Complete sequence of chromosome 1 of Acidovorax sp. JS42." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000539 Genomic DNA. Translation: ABM43886.1.
RefSeq	YP_987962.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1WCB1.
Genome annotation databases
GeneID	4671680.
GenomeReviews	Gene locus Ajs_3777 in contig CP000539_GR.
KEGG	ajs:Ajs_3777.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FYRDFLH.
Family and domain databases
HAMAP	MF_00747. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACEK_ACISJ

Accession

Primary (citable) accession number: A1WCB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)