Reviewed, UniProtKB/Swiss-Prot A1WC79 (PYRC_ACISJ)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	Ajs_3745

Organism

Acidovorax sp. (strain JS42) [Complete proteome] [HAMAP]

Taxonomic identifier

232721 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate. HAMAP MF_00219
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_00219
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3. HAMAP MF_00219
Subunit structure	Homodimer By similarity. HAMAP MF_00219
Sequence similarities	Belongs to the DHOase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Dihydroorotase HAMAP MF_00219		PRO_0000325566
Sites
	Metal binding	17	1	Zinc 1 By similarity
	Metal binding	19	1	Zinc 1 By similarity
	Metal binding	102	1	Zinc 1; via carbamate group By similarity
	Metal binding	102	1	Zinc 2; via carbamate group By similarity
	Metal binding	139	1	Zinc 2 By similarity
	Metal binding	177	1	Zinc 2 By similarity
	Metal binding	250	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	102	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1WC79-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 310D6DDF1B651282	FASTA	347	38,041
	10 20 30 40 50 60 MTATTDTLTI TRPDDWHLHV RDGEPLRTVV PHTAAQFGRA IIMPNLRPPV TTAQQAAEYK 70 80 90 100 110 120 QRILAAVPEG VAFEPLMTLY LTDNLPPDEI ARARDAGVVA AKLYPAGATT NSDAGVTDLR 130 140 150 160 170 180 KTYKTLEAMQ KAGLLLLVHG EVTSSDIDLF DREAVFIEQQ LIPLRRHFPE LKIVFEHITT 190 200 210 220 230 240 KEAAQYVKEA GRFTAATITA HHLLYNRNAI FTGGIRPHYY CLPVLKREVH RQALVQAATS 250 260 270 280 290 300 GSDKFFLGTD SAPHPAHLKE HATGCAGCYT AHAAIEMYAE AFDNAGALDK LEGFASVHGP 310 320 330 340 AFYGLPRNTG TVTLRRESWT PPDSFAFGEA ALKPLRAGEA LPWRLVA

References

[1]

"Complete sequence of chromosome 1 of Acidovorax sp. JS42." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000539 Genomic DNA. Translation: ABM43854.1.
RefSeq	YP_987930.1.
3D structure databases
SMR	A1WC79. Positions 5-345.
ModBase	Search...
Protein-protein interaction databases
STRING	A1WC79.
Genome annotation databases
GeneID	4672286.
GenomeReviews	Gene locus Ajs_3745 in contig CP000539_GR.
KEGG	ajs:Ajs_3745.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0418.
HOGENOM	HBG628648.
OMA	CLPVAKR.
PhylomeDB	A1WC79.
Family and domain databases
HAMAP	MF_00219. PyrC_type1. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004721. DHOdimr. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
PIRSF	PIRSF001237. DHOdimr. 1 hit.
TIGRFAMs	TIGR00856. pyrC_dimer. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_ACISJ

Accession

Primary (citable) accession number: A1WC79

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	February 6, 2007
Last modified:	February 9, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)