Reviewed, UniProtKB/Swiss-Prot A1WC05 (MURC_ACISJ)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	Ajs_3669

Organism

Acidovorax sp. (strain JS42) [Complete proteome] [HAMAP]

Taxonomic identifier

232721 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00046
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable HAMAP MF_00046.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 477	477	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_1000004304
Regions
	Nucleotide binding	112 – 118	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A1WC05-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 26D8A90DA179FCDD	FASTA	477	50,331
	10 20 30 40 50 60 MKQAIRHIHF VGIGGSGMCG IAEVLHNLGY VVSGSDLADS PTLRRLQSLG VATHVGHAAA 70 80 90 100 110 120 HIAGADAVVT STAVQADNPE VLAARERKIP VVPRALMLTE LMRLRKGIAI AGAHGKTTTT 130 140 150 160 170 180 SLVASVLGEA GLDPTFVIGG RLNSAGTNAK LGQGEYIVVE ADESDGSFLN LLPVMAVVTN 190 200 210 220 230 240 IDADHMETYG HDFGRLKQAF VDFLHRMPFY GTAILCIDNP AVRDILPLVT CPVTSYGLSE 250 260 270 280 290 300 DAEVRAVDVR AVGTQMHFTV QRRNGVTLPD LQVVLNLAGE HNVLNALAVI AVAAELNVPD 310 320 330 340 350 360 DALLRALAGF TGVGRRFQRH GDLPAQGGGH FTLIEDYGHH PVEMAATLAA ARGAYPGRRL 370 380 390 400 410 420 VLAFQPHRYS RTRDCFEDFV KVLGTADAVL LTEVYAAGEA PIVAADGRSL ARALRVAGTV 430 440 450 460 470 EPVFIDNVAD MPQRIAAGAR DGDVVLCMGA GSIGGVPAKV VDLLQKNELL AQEGRAQ

References

[1]

"Complete sequence of chromosome 1 of Acidovorax sp. JS42." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000539 Genomic DNA. Translation: ABM43780.1.
RefSeq	YP_987856.1.
3D structure databases
SMR	A1WC05. Positions 3-468.
ModBase	Search...
Protein-protein interaction databases
STRING	A1WC05.
Genome annotation databases
GeneID	4673644.
GenomeReviews	Gene locus Ajs_3669 in contig CP000539_GR.
KEGG	ajs:Ajs_3669.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0773.
HOGENOM	HBG476594.
OMA	YQPHRYS.
PhylomeDB	A1WC05.
Family and domain databases
HAMAP	MF_00046. MurC. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_ACISJ

Accession

Primary (citable) accession number: A1WC05

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	February 9, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)