Reviewed, UniProtKB/Swiss-Prot A1WBI7 (PANB_ACISJ)

Names and origin

Protein names

Recommended name:
    3-methyl-2-oxobutanoate hydroxymethyltransferase
    EC=2.1.2.11
Alternative name(s):
    Ketopantoate hydroxymethyltransferase
      Short name=KPHMT

Gene names

Name:	panB
Ordered Locus Names:	Ajs_3499

Organism

Acidovorax sp. (strain JS42) [Complete proteome] [HAMAP]

Taxonomic identifier

232721 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate. HAMAP MF_00156
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2. HAMAP MF_00156
Subunit structure	Homodecamer; pentamer of dimers By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the panB family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-methyl-2-oxobutanoate hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 293	293	3-methyl-2-oxobutanoate hydroxymethyltransferase HAMAP MF_00156		PRO_0000297207
Regions
	Region	67 – 68	2	Alpha-ketoisovalerate binding By similarity
Sites
	Active site	208	1	Proton acceptor By similarity
	Metal binding	67	1	Magnesium By similarity
	Metal binding	110	1	Magnesium By similarity
	Metal binding	141	1	Magnesium By similarity
	Binding site	110	1	Alpha-ketoisovalerate By similarity
	Binding site	139	1	Alpha-ketoisovalerate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1WBI7-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 7E8EC06100334083	FASTA	293	31,009
	10 20 30 40 50 60 MTDSPTAGTP YGTLPPASPL PQRRPVSLPR LAQMREAGEK ITMLTAYDAT FAAVADAAGV 70 80 90 100 110 120 ECILVGDSLG MVCQGLPSTV GVTLEHMAYH TASVARGLHR VQGTAWLIAD LPYGSYAESR 130 140 150 160 170 180 EQALRSACQL MQAGAHMVKL EGGGWTAPTV QFLVERGVPV CAHLGLTPQT VHALGGYRVQ 190 200 210 220 230 240 GKGDQAAQQL RRQALELQDA GAAMLVLEMV PTPLARDLTQ ALPRCHTIGI GAGSGTAGQV 250 260 270 280 290 LVMHDMLGIN LGKNPKFVHD FMRDAGSVRG AIEAYVQAVK QGRFPDDALH AWN

References

[1]

"Complete sequence of chromosome 1 of Acidovorax sp. JS42." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000539 Genomic DNA. Translation: ABM43612.1.
RefSeq	YP_987688.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1WBI7.
Genome annotation databases
GeneID	4672727.
GenomeReviews	Gene locus Ajs_3499 in contig CP000539_GR.
KEGG	ajs:Ajs_3499.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	YATPEQT.
Family and domain databases
HAMAP	MF_00156. [Tree]
InterPro	IPR003700. Pantoate_hydroxy_MeTrfase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
PANTHER	PTHR20881. Pantoate_transf. 1 hit.
Pfam	PF02548. Pantoate_transf. 1 hit. [Graphical view]
PIRSF	PIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
TIGRFAMs	TIGR00222. panB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANB_ACISJ

Accession

Primary (citable) accession number: A1WBI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)