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Protein

Malate dehydrogenase

Gene

mdh

Organism
Acidovorax sp. (strain JS42)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei95SubstrateUniRule annotation1
Binding sitei101SubstrateUniRule annotation1
Binding sitei108NADUniRule annotation1
Binding sitei115NADUniRule annotation1
Binding sitei134SubstrateUniRule annotation1
Binding sitei165SubstrateUniRule annotation1
Active sitei190Proton acceptorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 18NADUniRule annotation7
Nucleotide bindingi132 – 134NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:Ajs_2791
OrganismiAcidovorax sp. (strain JS42)
Taxonomic identifieri232721 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeAcidovorax
Proteomesi
  • UP000000645 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002923672 – 328Malate dehydrogenaseAdd BLAST327

Interactioni

Protein-protein interaction databases

STRINGi232721.Ajs_2791.

Structurei

3D structure databases

ProteinModelPortaliA1W9K7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D9Z. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000220953.
KOiK00024.
OrthoDBiPOG091H03R4.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1W9K7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKKPVRVAV TGAAGQIGYA LLFRIASGEM LGKDQPVILQ LLEIPDEKAQ
60 70 80 90 100
NALKGVIMEL EDCAFPLLAG IEAHSDPMTA FKDTDYALLV GARPRGPGME
110 120 130 140 150
RADLLAANAQ IFTAQGKALN AVASRNVKVL VVGNPANTNA YIAMKSAPDL
160 170 180 190 200
PAKNFTAMLR LDHNRAASQL AAKGGFKVGD IKKLTVWGNH SPTMYADYRF
210 220 230 240 250
ATVDGKSVKD AINDPAWYKD VFLPTVGKRG AAIIAARGLS SAASAANAAI
260 270 280 290 300
DHMRDWALGS KGEWVTMGVP SNGEYGIPAG IVFGFPVTTE NGEYKIVEGL
310 320
PIDAFSQECI DKTLAELQGE QDGVKHLL
Length:328
Mass (Da):34,838
Last modified:June 26, 2007 - v2
Checksum:iA02967E44E7FA9BD
GO

Sequence cautioni

The sequence ABM42932 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000539 Genomic DNA. Translation: ABM42932.1. Different initiation.
RefSeqiWP_041836045.1. NC_008782.1.

Genome annotation databases

EnsemblBacteriaiABM42932; ABM42932; Ajs_2791.
KEGGiajs:Ajs_2791.
PATRICi20690937. VBIAciSp27161_2989.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000539 Genomic DNA. Translation: ABM42932.1. Different initiation.
RefSeqiWP_041836045.1. NC_008782.1.

3D structure databases

ProteinModelPortaliA1W9K7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi232721.Ajs_2791.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABM42932; ABM42932; Ajs_2791.
KEGGiajs:Ajs_2791.
PATRICi20690937. VBIAciSp27161_2989.

Phylogenomic databases

eggNOGiENOG4105D9Z. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000220953.
KOiK00024.
OrthoDBiPOG091H03R4.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMDH_ACISJ
AccessioniPrimary (citable) accession number: A1W9K7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: June 26, 2007
Last modified: November 2, 2016
This is version 61 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.