Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot A1W9F9 (ASSY_ACISJ)

Last modified November 3, 2009. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Argininosuccinate synthase
    EC=6.3.4.5
Alternative name(s):
    Citrulline--aspartate ligase
Gene names
Name: argG
Ordered Locus Names: Ajs_2743
OrganismAcidovorax sp. (strain JS42) [Complete proteome] [HAMAP]
Taxonomic identifier232721 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Hide | Top

Protein attributes

Sequence length448 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

Hide | Top

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP MF_00581

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP MF_00581

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm Probable.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 2 subfamily.

Hide | Top

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processarginine biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 448448Argininosuccinate synthase HAMAP MF_00581
PRO_1000025410

Regions

Nucleotide binding17 – 259ATP By similarity

Sites

Binding site431ATP By similarity
Binding site991Citrulline By similarity
Binding site1291ATP; via amide nitrogen By similarity
Binding site1311Aspartate By similarity
Binding site1311ATP By similarity
Binding site1351Aspartate By similarity
Binding site1351Citrulline By similarity
Binding site1361Aspartate By similarity
Binding site1361ATP By similarity
Binding site1391Citrulline By similarity
Binding site1921Citrulline By similarity
Binding site1941ATP By similarity
Binding site2011Citrulline By similarity
Binding site2031Citrulline By similarity
Binding site2801Citrulline By similarity

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
A1W9F9-1 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 861CD628C346138A

FASTA44849,343
        10         20         30         40         50         60 
MATILQHLPV GQKVGIAFSG GLDTSAALRW MKNKGALPYA YTANLGQPDE ADYDEIPRKA 

        70         80         90        100        110        120 
MEYGAEKARL IDCRTQLAHE GIAAIQAGAF HISTGGITYF NTTPLGRAVT GTMLVAAMKE 

       130        140        150        160        170        180 
DDVHIWGDGS TFKGNDIERF YRYGLLTNPA LKIYKPWLDQ KFIDELGGRA EMSAFMTKEG 

       190        200        210        220        230        240 
FGYKMSAEKA YSTDSNMLGA THEAKDLEFL SSGIRIVNPI MGVAFWKPEV DVPAEEVSVT 

       250        260        270        280        290        300 
FDEGQPVAVN GKEIADPVEL FLELNRIGGR HGLGMSDQIE NRIIEAKSRG IYEAPGMALL 

       310        320        330        340        350        360 
HIAYERLVTG IHNEDTIEQY RLNGLKLGRL LYQGRWFDPQ AIMLRETAQR WVARAVTGTV 

       370        380        390        400        410        420 
TLELRRGNDY SILNTESPNL TYAPERLSME KVEDAPFSPL DRIGQLTMRN LDISDTRGKL 

       430        440 
GVYARAGLLS LGGNAALAQL EDGGAKKK

« Hide

Hide | Top

References

[1]"Complete sequence of chromosome 1 of Acidovorax sp. JS42."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Hide | Top

Cross-references

Sequence databases

CP000539 Genomic DNA. Translation: ABM42884.1.
RefSeqYP_986960.1.

3D structure databases

SMRA1W9F9. Positions 3-442.
ModBaseSearch...

Protein-protein interaction databases

STRINGA1W9F9.

Genome annotation databases

GeneID4673452.
GenomeReviewsGene locus Ajs_2743 in contig CP000539_GR.
KEGGajs:Ajs_2743.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAGGRKEMS.

Family and domain databases

HAMAPMF_00581.
[Tree]
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
[Graphical view]
PANTHERPTHR11587. Arginosuc_synth. 1 hit.
PfamPF00764. Arginosuc_synth. 2 hits.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Hide | Top

Entry information

Entry nameASSY_ACISJ
AccessionPrimary (citable) accession number: A1W9F9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents