A1W9F0 (SYE_ACISJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	Ajs_2734

Organism

Acidovorax sp. (strain JS42) [Complete proteome] [HAMAP]

Taxonomic identifier

232721 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_1000001865
Regions
	Motif	12 – 22	11	"HIGH" region HAMAP MF_00022_B
	Motif	244 – 248	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	247	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1W9F0 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 41E35274E64A6B06	FASTA	469	52,897
	10 20 30 40 50 60 MTQQQVRTRF APSPTGFIHL GNIRSALYPW AFARANDGVF ILRIEDTDLE RSTQASVDVI 70 80 90 100 110 120 LEGMSWLQLD HDEGPYYQMQ RMERYKEVLA QLQAAGHVYP CYMSVEELDA LRERQMAAKE 130 140 150 160 170 180 KPRYDGTWRP EPGKVLPPVP EGVKPVLRFK TPQGGVVGWD DKCKGRIEFQ NSELDDLVIA 190 200 210 220 230 240 RPDGTPTYNF CVCVDDMDMR ITHVIRGDDH VNNTPRQIHI FEALGATVPV FAHLPTVLNE 250 260 270 280 290 300 QGEKMSKRNG AKAVTQYRDE GYLPDAMVNY LARLGWSHGD DEIFSRAQFL EWFNLDHLGR 310 320 330 340 350 360 SAGQFDEAKL RWVNAQHLKA MEDAQLAALV RPFVVQAGVP ETQLDADDRL PRICALFKDR 370 380 390 400 410 420 CETLVDLARW ARVFYVDAIE PDADDFAKHV TETAAPALDA FAAAMETVAW NKDAINAAIK 430 440 450 460 DVLKQLGLKM PQLAMPVRVL TMGTAHTPSV DAVLELLGRE KILARLKSR

References

[1]

"Complete sequence of chromosome 1 of Acidovorax sp. JS42." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JS42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000539 Genomic DNA. Translation: ABM42875.1.
RefSeq	YP_986951.1. NC_008782.1.
3D structure databases
ProteinModelPortal	A1W9F0.
ModBase	Search...
Protein-protein interaction databases
STRING	A1W9F0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4673089.
GenomeReviews	Gene locus Ajs_2734 in contig CP000539_GR.
KEGG	ajs:Ajs_2734.
PATRIC	20690819. VBIAciSp27161_2931.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	VRFRMPD.
PhylomeDB	A1W9F0.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	ASP232721:AJS_2734-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_ACISJ

Accession

Primary (citable) accession number: A1W9F0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program