A1W8I6 (SYT_ACISJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	Ajs_2400

Organism

Acidovorax sp. (strain JS42) [Complete proteome] [HAMAP]

Taxonomic identifier

232721 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	639 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00184
Subunit structure	Homodimer By similarity. HAMAP MF_00184
Subcellular location	Cytoplasm HAMAP MF_00184.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 639	639	Threonine--tRNA ligase HAMAP MF_00184		PRO_1000020331
Regions
	Region	242 – 533	292	Catalytic HAMAP MF_00184
Sites
	Metal binding	333	1	Zinc; catalytic By similarity
	Metal binding	384	1	Zinc; catalytic By similarity
	Metal binding	510	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1W8I6 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: F5AF692876C862AD	FASTA	639	72,156
	10 20 30 40 50 60 MIHITLPDGS QREFAGPVTV AEVAASIGSG LAKAALAGKI GDKVVDTSYQ ITQDSPLSIV 70 80 90 100 110 120 TAKDADGLEV IRHSTAHLLA YAVKELFPDA QVTIGPVIEN GFYYDFSYKR PFTPEDLAAI 130 140 150 160 170 180 EKRMAELAAK DEPVVRRVLP RDEAVAYFKG LGEHYKAEII ASIPTNEDVS LYREGGFEDL 190 200 210 220 230 240 CRGPHVPSTG KLKFFKLMKV AGAYWRGDHR NEMLQRIYGT AWATKEELQE YLHMLEEAEK 250 260 270 280 290 300 RDHRKLGREL DLFHIDEHSP GTVFWHPKGW TLWQEVEQYM RRVYRDNGYQ EVKGPQILDK 310 320 330 340 350 360 TLWEKTGHWD KYRDNMFTTE SEKRDYALKP MNCPGHILIF KQGIKSYRDL PLRYGEFGQC 370 380 390 400 410 420 HRNEPTGGLH GIMRVRGFTQ DDGHIFCTED HILAECTAYT ALLQKVYKDF GFHNIIYKVA 430 440 450 460 470 480 TRPEARIGSD ESWDKAEHAL MESLRASGCE FEIAPGDGAF YGPKIEYTLK DAIGRQWQCG 490 500 510 520 530 540 TMQVDFSMPE RLDAEYVGED GGRHRPVMLH RAIVGSLERF IGILIEEHAG ALPVWLAPVQ 550 560 570 580 590 600 VAVLNITDAQ QDYCREIAAK LQKALPNQGL RVVTDLRNEK ITYKIREHSL QKLPYILVAG 610 620 630 DKEKAAGAVA VRARGNKDLG VMSLDAFVDL IAQDIASKV

References

[1]

"Complete sequence of chromosome 1 of Acidovorax sp. JS42." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JS42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000539 Genomic DNA. Translation: ABM42561.1.
RefSeq	YP_986637.1. NC_008782.1.
3D structure databases
ProteinModelPortal	A1W8I6.
SMR	A1W8I6. Positions 241-639.
ModBase	Search...
Protein-protein interaction databases
STRING	A1W8I6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4671495.
GenomeReviews	Gene locus Ajs_2400 in contig CP000539_GR.
KEGG	ajs:Ajs_2400.
PATRIC	20690167. VBIAciSp27161_2612.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0441.
HOGENOM	HBG352811.
OMA	VGSDALW.
PhylomeDB	A1W8I6.
ProtClustDB	PRK00413.
Enzyme and pathway databases
BioCyc	ASP232721:AJS_2400-MONOMER.
Family and domain databases
HAMAP	MF_00184. Thr_tRNA_synth. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR012675. Beta-grasp_ferredoxin-type. IPR004095. TGS. IPR012676. TGS-like. IPR002320. Thr-tRNA-synth_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
KO	K01868.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF81271. TGS-like. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. ThrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_ACISJ

Accession

Primary (citable) accession number: A1W8I6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program