Reviewed, UniProtKB/Swiss-Prot A1W7S6 (PYRD_ACISJ)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.5.2
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	Ajs_2132

Organism

Acidovorax sp. (strain JS42) [Complete proteome] [HAMAP]

Taxonomic identifier

232721 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + a quinone = orotate + a quinol. HAMAP MF_00225
Cofactor	Binds 1 FMN per subunit By similarity. HAMAP MF_00225
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1. HAMAP MF_00225
Subunit structure	Homodimer By similarity. HAMAP MF_00225
Subcellular location	Cell membrane; Peripheral membrane protein By similarity HAMAP MF_00225.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Dihydroorotate dehydrogenase HAMAP MF_00225		PRO_1000024145
Sites
	Active site	186	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1W7S6-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 25A3529D16DFA89D	FASTA	351	37,431
	10 20 30 40 50 60 MSLLPYALAR SFLFGMDAEA AHELTMDMLA RGQRTPLQWA WCNETVSDPI ELAGLRFPNR 70 80 90 100 110 120 VGLAAGLDKN ARCIDALAAM GFGFVEVGTV TPRAQPGNPK PRMFRLPEAR ALINRLGFNN 130 140 150 160 170 180 EGLDAFVANV QRSQVRTQGR GQSKLLLGLN IGKNATTPIE DATRDYLTCL EGVYPHADYV 190 200 210 220 230 240 TVNISSPNTQ NLRALQSDAA LDGLLGAIAE HREQLAAAQG RRVPIFVKIA PDLDEAQVAV 250 260 270 280 290 300 IATTLQRHGM DGVVATNTTI RRDAVQGLRH AGETGGLSGA PVLEASNAVI RQLRAALGPT 310 320 330 340 350 FPIIGVGGIL SAEDAVSKIR AGADVVQIYT GLIYEGPALV GRAAKAIRDL R

References

[1]

"Complete sequence of chromosome 1 of Acidovorax sp. JS42." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000539 Genomic DNA. Translation: ABM42301.1.
RefSeq	YP_986377.1.
3D structure databases
SMR	A1W7S6. Positions 4-345.
ModBase	Search...
Protein-protein interaction databases
STRING	A1W7S6.
Genome annotation databases
GeneID	4670618.
GenomeReviews	Gene locus Ajs_2132 in contig CP000539_GR.
KEGG	ajs:Ajs_2132.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0167.
HOGENOM	HBG351027.
OMA	NAEQQGG.
PhylomeDB	A1W7S6.
Family and domain databases
HAMAP	MF_00225. DHO_dh_type2. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005719. Dihydroorotate_DH_2. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_ACISJ

Accession

Primary (citable) accession number: A1W7S6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	February 9, 2010
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)