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A1W7D2 (TRMB_ACISJ) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:Ajs_1981
OrganismAcidovorax sp. (strain JS42) [Complete proteome] [HAMAP]
Taxonomic identifier232721 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeAcidovorax

Protein attributes

Sequence length251 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 251251tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288113

Regions

Region165 – 1706Interaction with RNA Potential
Region230 – 2334Substrate binding By similarity

Sites

Active site1591 By similarity
Binding site841S-adenosyl-L-methionine By similarity
Binding site1091S-adenosyl-L-methionine By similarity
Binding site1361S-adenosyl-L-methionine By similarity
Binding site1591S-adenosyl-L-methionine By similarity
Binding site1631Substrate By similarity
Binding site1951Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A1W7D2 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 1A6DC5AB16355179

FASTA25127,506
        10         20         30         40         50         60 
MTQTLSSQDP QAPAAPPMPG AAGSAPADVA HPKGIKSYVR RAGRTTTGQA KALEELGPRY 

        70         80         90        100        110        120 
VLDYAPALLD ATAAFGRSAP LVLEIGFGMG EATAHIARVR PQDNFLCCEV HEPGVGALLK 

       130        140        150        160        170        180 
RIGEQDIHNI RILQHDAVEV LEHMLAPGAL DGVHIFFPDP WHKKRHNKRR LIQPPLVAQL 

       190        200        210        220        230        240 
AARLKPGGYI HCATDWQPYA EQMLEVLSAE PLLSNTAEGY APQPDYRPLT KFENRGMRLG 

       250 
HGVWDLVFTR R 

« Hide

References

[1]"Complete sequence of chromosome 1 of Acidovorax sp. JS42."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JS42.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000539 Genomic DNA. Translation: ABM42157.1.
RefSeqYP_986233.1. NC_008782.1.

3D structure databases

ProteinModelPortalA1W7D2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING232721.Ajs_1981.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM42157; ABM42157; Ajs_1981.
GeneID4674401.
KEGGajs:Ajs_1981.
PATRIC20689292. VBIAciSp27161_2180.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMAQDNFLCC.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycASP232721:GHWE-2006-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_ACISJ
AccessionPrimary (citable) accession number: A1W7D2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: February 6, 2007
Last modified: June 11, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways