Reviewed, UniProtKB/Swiss-Prot A1W7D0 (SYA_ACISJ)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Ajs_1979

Organism

Acidovorax sp. (strain JS42) [Complete proteome] [HAMAP]

Taxonomic identifier

232721 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	874 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Domain	Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 874	874	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000347472
Regions
	Zinc finger	180 – 193	14	C2H2-type HAMAP MF_00036

Sequences

	Sequence		Length	Mass (Da)
	A1W7D0-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: E37B8D88D7301B45	FASTA	874	94,392
	10 20 30 40 50 60 MSTPTFSVAD IRKTFLDFFA AKGHTVVASS PLVPGNDPTL MFTNSGMVQF KDVFLGTDKR 70 80 90 100 110 120 PYVRATSVQT CLRAGGKHND LENVGYTARH HTFFEMLGNW SFGDYFKRES LKWAWELLTE 130 140 150 160 170 180 VYKLPPERLL ATVYAEDDEA YDIWTKEIGL PPERVIRIGD NKGGRYKSDN FWMMADTGPC 190 200 210 220 230 240 GPCSEIFYDH GEHIAGGPPG SPDEDGDRFI EIWNNVFMQF DMDEQGNVKP LPAPCVDTGM 250 260 270 280 290 300 GLERLAAILQ HVHSNYEIDL FDALIKAAAR ETGTSDLTNP SLKVIADHIR ATAFLVADGV 310 320 330 340 350 360 IPSNEGRGYV QRRIVRRAIR HGYKLGRKTP FFHKLVQDLV QQMGDAYPKI REQQARITDV 370 380 390 400 410 420 LRVEEERFFE TLAHGMEILD SALAGGAKTL PGDVAFKLHD TYGFPLDLTN DVCRERGVNV 430 440 450 460 470 480 DEAGFATAME HQKSTARAAG KFKMDRALEY TGAANQFTGY EQLAESAKIV ALYVDGTSTA 490 500 510 520 530 540 ALHAGQSGVV VLDRTPFYAE SGGQVGDQGT IGAGSACFTV ADTQKIKADV YGHHGTLEAG 550 560 570 580 590 600 TLNVGDTVQA QVDLQLRAAT MRNHSVTHLM HKALREVLGD HVQQKGSLVN AERTRFDFAH 610 620 630 640 650 660 NAPLTAAQIR EIERLVNAEV LANTDTNARL MDIESAQKTG AMMLFGEKYG ETVRVLDIGT 670 680 690 700 710 720 SRELCGGTHV RRTGDIGLFK VVAEGGVAAG VRRIEAVTGE NALAYLQSLE STVDQAAAAL 730 740 750 760 770 780 KAPPAELTAR IGGALDQIKT LEKELAALKG KLASSQGDEL AGQAVDVKGI KVLAARLEGA 790 800 810 820 830 840 DAKTLRETMD KLKDKLKTAA IVLAAVDGDK VQLAAGVTAD SIGRVKAGDL VNFVAAQVGG 850 860 870 KGGGKPDMAM AGGTNAAALP QALAAVQGWV GERI

References

[1]

"Complete sequence of chromosome 1 of Acidovorax sp. JS42." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000539 Genomic DNA. Translation: ABM42155.1.
RefSeq	YP_986231.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1W7D0.
Genome annotation databases
GeneID	4674560.
GenomeReviews	Gene locus Ajs_1979 in contig CP000539_GR.
KEGG	ajs:Ajs_1979.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	TLMFTNS.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_ACISJ

Accession

Primary (citable) accession number: A1W7D0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)