A1W6E1 (SYC_ACISJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	Ajs_1623

Organism

Acidovorax sp. (strain JS42) [Complete proteome] [HAMAP]

Taxonomic identifier

232721 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	458 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 458	458	Cysteine--tRNA ligase HAMAP MF_00041		PRO_0000332779
Regions
	Motif	31 – 41	11	"HIGH" region HAMAP MF_00041
	Motif	270 – 274	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	29	1	Zinc By similarity
	Metal binding	213	1	Zinc By similarity
	Metal binding	238	1	Zinc By similarity
	Metal binding	242	1	Zinc By similarity
	Binding site	273	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1W6E1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: CAFEE1E186EA1706	FASTA	458	50,126
	10 20 30 40 50 60 MSLRIYNTLS RALEEFSPLE PGHVRMYVCG MTVYDLCHLG HARSMIAFDV VQRWLRASGL 70 80 90 100 110 120 AVTYVRNITD IDDKIIKRAV ENGETIRSLT DRMIDALHQD ADALGIERPT HEPRATAYVP 130 140 150 160 170 180 QMLDMIGTLQ GKGLAYQAGN GDVNYAVRKF PGYGKLSGKS LDELNAGERV AVQDGKHDPL 190 200 210 220 230 240 DFVLWKSAKP EEPADVKWRS PFGEGRPGWH IECSAMGCAL LGESFDIHGG GADLQFPHHE 250 260 270 280 290 300 NEIAQSEGAT GKPFARLWMH NGFINVDNEK MSKSLGNFFT IRDVLKEYDA ETVRFFVVRS 310 320 330 340 350 360 HYRSPLNYSN VHLDDARAAL KRLYTALSLV APAPVEVDWA EGYAARFKAA MDEDFGTPEA 370 380 390 400 410 420 VAVLFDLAGE VNRSKSPAAA GLLKALGGHL GLLQADPQDF LKAGAGLDEA AIQAQIAARA 430 440 450 TAKAAKNFAE ADRIRNDLLA QGIVLKDSAS GTTWEAAQ

References

[1]

"Complete sequence of chromosome 1 of Acidovorax sp. JS42." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JS42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000539 Genomic DNA. Translation: ABM41816.1.
RefSeq	YP_985892.1. NC_008782.1.
3D structure databases
ProteinModelPortal	A1W6E1.
SMR	A1W6E1. Positions 3-401.
ModBase	Search...
Protein-protein interaction databases
STRING	A1W6E1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4674710.
GenomeReviews	Gene locus Ajs_1623 in contig CP000539_GR.
KEGG	ajs:Ajs_1623.
PATRIC	20688564. VBIAciSp27161_1821.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0215.
HOGENOM	HBG327651.
OMA	DFDALNM.
PhylomeDB	A1W6E1.
ProtClustDB	PRK00260.
Enzyme and pathway databases
BioCyc	ASP232721:AJS_1623-MONOMER.
Family and domain databases
HAMAP	MF_00041. Cys_tRNA_synth. [Tree]
InterPro	IPR015803. Cys-tRNA-synt. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_ACISJ

Accession

Primary (citable) accession number: A1W6E1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program