Reviewed, UniProtKB/Swiss-Prot A1W5A4 (TYSY_ACISJ)

Names and origin

Protein names

Recommended name:
    Thymidylate synthase
      Short name=TS
      Short name=TSase
    EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	Ajs_1197

Organism

Acidovorax sp. (strain JS42) [Complete proteome] [HAMAP]

Taxonomic identifier

232721 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity. HAMAP MF_00008
Subcellular location	Cytoplasm By similarity HAMAP MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type thyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	Thymidylate synthase HAMAP MF_00008		PRO_0000321464
Sites
	Active site	152	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1W5A4-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: B3589C202F79D389	FASTA	277	31,748
	10 20 30 40 50 60 MTPRPIRHQY EDLMRHVFEH GTAKGDRTGT GTKSVFGHQM RFDLSEGFPL VTTKKVHLKS 70 80 90 100 110 120 IILELLWFLR GDSNVRWLQE RGCTIWDEWA RDDGSLGPVY GVQWRSWPTP DGGHIDQIQQ 130 140 150 160 170 180 VIDTLKTHPD SRRIIVSAWN VAELDRMALM PCHAFFQFYV APSQVAGEPG KLSCQLYQRS 190 200 210 220 230 240 ADIFLGVPFN IASYALLTHM VAQQCDLQVG DFIWTGGDCH IYSNHFEQVR TQLARQPYAY 250 260 270 PTLQIKRRPA SIFDYAYEDF EVVGYEHHPA IKAPVAV

References

[1]

"Complete sequence of chromosome 1 of Acidovorax sp. JS42." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000539 Genomic DNA. Translation: ABM41429.1.
RefSeq	YP_985505.1.
3D structure databases
SMR	A1W5A4. Positions 9-277.
ModBase	Search...
Protein-protein interaction databases
STRING	A1W5A4.
Genome annotation databases
GeneID	4672547.
GenomeReviews	Gene locus Ajs_1197 in contig CP000539_GR.
KEGG	ajs:Ajs_1197.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0207.
HOGENOM	HBG588098.
OMA	RMALAPC.
PhylomeDB	A1W5A4.
Family and domain databases
HAMAP	MF_00008. Thymidy_synth_bact. [Tree]
InterPro	IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_ACISJ

Accession

Primary (citable) accession number: A1W5A4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	February 6, 2007
Last modified:	February 9, 2010
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)