A1W588 (PURA_ACISJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenylosuccinate synthetase
Short name=AMPSase
Short name=AdSS
EC=6.3.4.4

Alternative name(s):
IMP--aspartate ligase

Gene names

Name:	purA
Ordered Locus Names:	Ajs_1181

Organism

Acidovorax sp. (strain JS42) [Complete proteome] [HAMAP]

Taxonomic identifier

232721 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	458 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP By similarity. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00011
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity. HAMAP MF_00011
Subcellular location	Cytoplasm By similarity HAMAP MF_00011.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylosuccinate synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 458	458	Adenylosuccinate synthetase HAMAP MF_00011		PRO_0000321791
Regions
	Nucleotide binding	17 – 23	7	GTP By similarity
	Nucleotide binding	45 – 47	3	GTP By similarity
	Nucleotide binding	358 – 360	3	GTP By similarity
	Nucleotide binding	440 – 442	3	GTP By similarity
	Region	18 – 21	4	IMP binding By similarity
	Region	43 – 46	4	IMP binding By similarity
	Region	326 – 332	7	Substrate binding By similarity
Sites
	Active site	18	1	Proton acceptor By similarity
	Active site	46	1	Proton donor By similarity
	Metal binding	18	1	Magnesium By similarity
	Metal binding	45	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	137	1	IMP By similarity
	Binding site	151	1	IMP; shared with dimeric partner By similarity
	Binding site	247	1	IMP By similarity
	Binding site	262	1	IMP By similarity
	Binding site	330	1	IMP By similarity
	Binding site	332	1	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1W588 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: ED10C6FCD2345D5F	FASTA	458	49,378
	10 20 30 40 50 60 MKASKGRNVV VVGTQWGDEG KGKLVDWLTE SAQGVVRFQG GHNAGHTLVI NGVKTALHLI 70 80 90 100 110 120 PSGIMRPGVK CYIGNGVVLS AGKLFEEIEG LEKAGVEVRS RLRVSEACPL ILPFHAALDV 130 140 150 160 170 180 AREAAREHGG AEKIGTTGRG IGPAYEDKIA RRALRVQDLK YPERFAAKLR ELLALHNHVL 190 200 210 220 230 240 STFLGSANFQ FGDALKPYIT GGQVQFEPVF EEAMRHAEML KPMMADVSRE LNEAHAAGAN 250 260 270 280 290 300 LLFEGAQGTL LDVDHGTYPY VTSSNCVAGN AAAGSGVGPG HLHYILGITK AYCTRVGGGP 310 320 330 340 350 360 FPTELDWETP GTPGYHMSTV GAEKGVTTGR SRRCGWFDAA LLKRSAQVNG LSGLCITKLD 370 380 390 400 410 420 VLDGLRELSL CVGYELDGER IDLLPMGAEE IARCVPIYEN IAGWTESTVG VTHYENLPSN 430 440 450 ARRYLERIEQ VTGVPIAMIS TSPDRDHTIL MRHPYAAD

References

[1]

"Complete sequence of chromosome 1 of Acidovorax sp. JS42." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JS42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000539 Genomic DNA. Translation: ABM41413.1.
RefSeq	YP_985489.1. NC_008782.1.
3D structure databases
ProteinModelPortal	A1W588.
SMR	A1W588. Positions 6-457.
ModBase	Search...
Protein-protein interaction databases
STRING	A1W588.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4672451.
GenomeReviews	Gene locus Ajs_1181 in contig CP000539_GR.
KEGG	ajs:Ajs_1181.
PATRIC	20687670. VBIAciSp27161_1386.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0104.
HOGENOM	HBG658237.
OMA	YVLGIIK.
PhylomeDB	A1W588.
ProtClustDB	PRK01117.
Enzyme and pathway databases
BioCyc	ASP232721:AJS_1181-MONOMER.
Family and domain databases
HAMAP	MF_00011. Adenylosucc_synth. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
KO	K01939.
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. PurA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA_ACISJ

Accession

Primary (citable) accession number: A1W588

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program