Reviewed, UniProtKB/Swiss-Prot A1W4U9 (DXS_ACISJ)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose-5-phosphate synthase
    EC=2.2.1.7
Alternative name(s):
    1-deoxyxylulose-5-phosphate synthase
      Short name=DXP synthase
      Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	Ajs_1038

Organism

Acidovorax sp. (strain JS42) [Complete proteome] [HAMAP]

Taxonomic identifier

232721 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	629 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity. HAMAP MF_00315
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP thiamin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 629	629	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000018999

Sequences

	Sequence		Length	Mass (Da)
	A1W4U9-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: ED087D75A2887CC7	FASTA	629	67,633
	10 20 30 40 50 60 MSMTNYPLLE RVNDPADLRR LPRAELKTLA HELRAFVLES VSKTGGHLSS NLGTVELTVA 70 80 90 100 110 120 LHAVFDTPRD RLVWDVGHQT YPHKILTGRR DRMPSLRQLG GLSGFPQRAE SEYDTFGTAH 130 140 150 160 170 180 SSTSISAALG MALAAKQRGD ERRCVAIIGD GAMTAGMAFE ALNNAGVADA NLLVILNDND 190 200 210 220 230 240 MSISPPVGAL NRYLAQLMSG QFYAKARDMG KSVLKNAPPL LELAKRLEQQ AKGMVVPATL 250 260 270 280 290 300 FEKFGFNYIG PIDGHDLDSL IPTLENIRGL KGPQFLHVVT KKGQGYKLAE ADPVAYHGPG 310 320 330 340 350 360 KFDPRVGLVK PATPPKQTFT QVFGQWLCDM ADKDKRLVGI TPAMREGSGM VEFHQRFPDR 370 380 390 400 410 420 YYDVGIAEQH AVTFAAGMAC EGVKPVVAIY STFLQRAYDQ LIHDVALQNL PVVFALDRAG 430 440 450 460 470 480 LVGADGATHA GAYDIAFVRC IPHMSMACPA DERECRQLLT TAYEQDHPVA VRYPRGAGVG 490 500 510 520 530 540 VAPLPDLEGL PFGKGEVRRT GQRIAILAFG TLLYPALQAA QALDATVVNM RWAKPLDTQL 550 560 570 580 590 600 LLQVAAEHDA LVTVEEGCIM GGAGSAVAEA LAAAGLQRPL LQLGLPDVFI EHGDPAKLLA 610 620 LQGLDAAGMQ RSITERFGAL PGAQALAAA

References

[1]

"Complete sequence of chromosome 1 of Acidovorax sp. JS42." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000539 Genomic DNA. Translation: ABM41274.1.
RefSeq	YP_985350.1.
3D structure databases
SMR	A1W4U9. Positions 2-615.
ModBase	Search...
Protein-protein interaction databases
STRING	A1W4U9.
Genome annotation databases
GeneID	4670731.
GenomeReviews	Gene locus Ajs_1038 in contig CP000539_GR.
KEGG	ajs:Ajs_1038.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1154.
HOGENOM	HBG571647.
OMA	QRFPDRY.
PhylomeDB	A1W4U9.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR001017. DH_E1. IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
TIGRFAMs	TIGR00204. dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_ACISJ

Accession

Primary (citable) accession number: A1W4U9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	February 9, 2010
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)