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A1W4S3 (DAPA_ACISJ) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52
Gene names
Name:dapA
Ordered Locus Names:Ajs_1010
OrganismAcidovorax sp. (strain JS42) [Complete proteome] [HAMAP]
Taxonomic identifier232721 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length298 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418

Subunit structure

Homotetramer By similarity. HAMAP MF_00418

Subcellular location

Cytoplasm By similarity HAMAP MF_00418.

Sequence similarities

Belongs to the DHDPS family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Diaminopimelate biosynthesis
Lysine biosynthesis
   Cellular componentCytoplasm
   LigandSchiff base
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processdiaminopimelate biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiondihydrodipicolinate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 298298Dihydrodipicolinate synthase HAMAP MF_00418
PRO_0000340931

Regions

Region54 – 552Pyruvate binding By similarity

Sites

Active site1681Schiff-base intermediate with substrate By similarity
Binding site1121Pyruvate By similarity
Site1401Involved in proton transfer during cleavage By similarity

Sequences

Sequence LengthMass (Da)Tools
A1W4S3 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 587181FF5B1126D5

FASTA29831,854
        10         20         30         40         50         60 
MTSPSAPLTG SIVALVTPMH DDGSVDYPAL RKLIDWHIAE GTDCIGVVGT TGESPTVNVE 

        70         80         90        100        110        120 
EHCEIIRVSV EQAAKRVPIM AGCGANSTAE AIELARFAKK VGADSQLQVV PYYNKPTQEG 

       130        140        150        160        170        180 
QYRHFKAIAE AVGDLPMVLY NVPGRSVADM QHETVLRLTQ VPGIVGIKEA TGNIERAQWL 

       190        200        210        220        230        240 
IRDVPKGFAV YSGDDPTAVA LMLCGGQGNI SVTANVAPRL MHELCVAALA GDTRRAMEIQ 

       250        260        270        280        290 
FRLMPVHKQL FVEANPIPVK WAVQRMGLCG GALRLPMTPL SQGNEAVVEA ALRAAGLL

« Hide

References

[1]"Complete sequence of chromosome 1 of Acidovorax sp. JS42."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JS42.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000539 Genomic DNA. Translation: ABM41248.1.
RefSeqYP_985324.1. NC_008782.1.

3D structure databases

ProteinModelPortalA1W4S3.
SMRA1W4S3. Positions 8-298.
ModBaseSearch...

Protein-protein interaction databases

STRINGA1W4S3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4673524.
GenomeReviewsGene locus Ajs_1010 in contig CP000539_GR.
KEGGajs:Ajs_1010.
PATRIC20687298. VBIAciSp27161_1201.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0329.
HOGENOMHBG358848.
OMAFMLCGGH.
PhylomeDBA1W4S3.
ProtClustDBPRK03170.

Enzyme and pathway databases

BioCycASP232721:AJS_1010-MONOMER.

Family and domain databases

HAMAPMF_00418. DapA.
[Tree]
InterProIPR013785. Aldolase_TIM.
IPR002220. Dihydrodipicolinate_synth-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR005263. Dihydrodipicolinate_synth_DapA.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK01714.
PANTHERPTHR12128. DHDPS. 1 hit.
PfamPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFPIRSF001365. DHDPS. 1 hit.
PRINTSPR00146. DHPICSNTHASE.
TIGRFAMsTIGR00674. DapA. 1 hit.
PROSITEPS00665. DHDPS_1. False negative.
PS00666. DHDPS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDAPA_ACISJ
AccessionPrimary (citable) accession number: A1W4S3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: February 6, 2007
Last modified: December 14, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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