A1W4B1 (PROB_ACISJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate 5-kinase
EC=2.7.2.11

Alternative name(s):
Gamma-glutamyl kinase
Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	Ajs_0844

Organism

Acidovorax sp. (strain JS42) [Complete proteome] [HAMAP]

Taxonomic identifier

232721 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity HAMAP MF_00456.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 380	380	Glutamate 5-kinase HAMAP MF_00456		PRO_1000125208
Regions
	Domain	285 – 363	79	PUA

Sequences

	Sequence		Length	Mass (Da)
	A1W4B1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 3D1261B97FD3FFEA	FASTA	380	40,391
	10 20 30 40 50 60 MVSSVLRDAR RIVVKVGSSL VTNEGRGLDE VAIGEWCRQL AALVRGQGGE PREVIMVSSG 70 80 90 100 110 120 AIAEGMKRLG WSSRPGEIHE LQAAAAVGQM GLAQMYETKL REQSMGSAQV LLTHADLADR 130 140 150 160 170 180 ERYLNARSTL LTLLRLGVVP VINENDTVVT DEIKFGDNDT LGALVANLVE ADALVILTDQ 190 200 210 220 230 240 KGLYTADPRR DPQAQFVHEA QAGDAALEAM AGGAGSSIGK GGMITKILAA KRAAGSGAST 250 260 270 280 290 300 VIAWGREPDV LLRLARGESI GTLLVAQTQK NQARKQWMVD HLQLRGSVTV DAGAAAKLRE 310 320 330 340 350 360 DGKSLLPIGM VAVEGDFVRG DVIAVRDPGG TEIARGLANY ASAEARLLCR KPSAEFERLL 370 380 GYAAEPEMVH RDNMVVLGAR

References

[1]

"Complete sequence of chromosome 1 of Acidovorax sp. JS42." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JS42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000539 Genomic DNA. Translation: ABM41086.1.
RefSeq	YP_985162.1. NC_008782.1.
3D structure databases
ProteinModelPortal	A1W4B1.
ModBase	Search...
Protein-protein interaction databases
STRING	A1W4B1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4673260.
GenomeReviews	Gene locus Ajs_0844 in contig CP000539_GR.
KEGG	ajs:Ajs_0844.
PATRIC	20686954. VBIAciSp27161_1032.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0263.
HOGENOM	HBG507643.
OMA	TFGDNDM.
PhylomeDB	A1W4B1.
ProtClustDB	PRK05429.
Enzyme and pathway databases
BioCyc	ASP232721:AJS_0844-MONOMER.
Family and domain databases
HAMAP	MF_00456. ProB. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. IPR011529. Glu_5kinase. IPR005715. Glu_5kinase/COase_Synthase. IPR019797. Glutamate_5-kinase_CS. IPR002478. PUA. IPR015947. PUA-like_domain. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00931.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF88697. PUA-like. 1 hit.
TIGRFAMs	TIGR01027. ProB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_ACISJ

Accession

Primary (citable) accession number: A1W4B1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program