Reviewed, UniProtKB/Swiss-Prot A1W2F6 (ALR_ACISJ)

Names and origin

Protein names

Recommended name:
    Alanine racemase
    EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	Ajs_0176

Organism

Acidovorax sp. (strain JS42) [Complete proteome] [HAMAP]

Taxonomic identifier

232721 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: HAMAP cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	Alanine racemase HAMAP MF_01201		PRO_1000065966
Sites
	Active site	35	1	Proton acceptor; specific for D-alanine By similarity
	Active site	256	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	35	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1W2F6-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 6B5E45547282E80E	FASTA	365	39,386
	10 20 30 40 50 60 MPRPILATIH PAAVHHNLER ARRAAPDARV WAVVKANAYG HGIERVFEGL RAADGFALLD 70 80 90 100 110 120 LAEAERVRAL GWRGPILLLE GVFEPRDLEL CSRLGLWHAV HCDAQIDWLA AHKTQVPHRV 130 140 150 160 170 180 FLKMNSGMNR LGFTPERYRS AWARLNALPQ VDEISCMTHF SDADGPRGIA HQVQAFQAAT 190 200 210 220 230 240 QDLPGERCIA NSAALLRHGG DAQVRLDWVR AGIVLYGSAP DHPERRAADW DLQPTMTLAS 250 260 270 280 290 300 RIIGVQQLQA GDTVGYGSRF TAQGPLGIGI VACGYADGYP RHCDTGTPVL VNGVRTRTIG 310 320 330 340 350 360 RVSMDMLAVD LTPVPGAGLG AEVTLWGRAA NGAVLPIDEV AQAGGTIGYE LMCALAPRVP VVVQD

References

[1]

"Complete sequence of chromosome 1 of Acidovorax sp. JS42." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000539 Genomic DNA. Translation: ABM40431.1.
RefSeq	YP_984507.1.
3D structure databases
SMR	A1W2F6. Positions 3-362.
ModBase	Search...
Protein-protein interaction databases
STRING	A1W2F6.
Genome annotation databases
GeneID	4670888.
GenomeReviews	Gene locus Ajs_0176 in contig CP000539_GR.
KEGG	ajs:Ajs_0176.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0787.
HOGENOM	HBG712172.
OMA	FFHADEL.
PhylomeDB	A1W2F6.
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
TIGRFAMs	TIGR00492. alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_ACISJ

Accession

Primary (citable) accession number: A1W2F6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 6, 2007
Last modified:	February 9, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)