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Protein

2-isopropylmalate synthase

Gene

leuA

Organism
Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).UniRule annotation

Catalytic activityi

Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. 2-isopropylmalate synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Enzyme and pathway databases

BioCyciCJEJ354242:GC51-1698-MONOMER.
UniPathwayiUPA00048; UER00070.

Names & Taxonomyi

Protein namesi
Recommended name:
2-isopropylmalate synthaseUniRule annotation (EC:2.3.3.13UniRule annotation)
Alternative name(s):
Alpha-IPM synthaseUniRule annotation
Alpha-isopropylmalate synthaseUniRule annotation
Gene namesi
Name:leuAUniRule annotation
Ordered Locus Names:CJJ81176_0017
OrganismiCampylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176)
Taxonomic identifieri354242 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000000646 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5115112-isopropylmalate synthasePRO_1000149163Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi354242.CJJ81176_0017.

Structurei

3D structure databases

ProteinModelPortaliA1W1X2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0119.
HOGENOMiHOG000046859.
KOiK01649.
OMAiWSVHCHN.
OrthoDBiEOG6CGCF3.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01025. LeuA_type1.
InterProiIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005671. LeuA_bact_synth.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTiSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF110921. SSF110921. 1 hit.
TIGRFAMsiTIGR00973. leuA_bact. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1W1X2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDNKIIIFD TTLRDGEQAL GSSLGINQKL QIALALENLG VDVIEAGFPV
60 70 80 90 100
SSQGDFKAVQ KIASKVKNST ICALSRALDK DIDMAYEALK VAKHFRIHTF
110 120 130 140 150
IATSTLHMQD KLKKDFDEIL SMAKRAIIRA RSYTDDVEFS CEDAGRTPID
160 170 180 190 200
NLCFMVENAI KAGAKTINIP DTVGYTLPSE FANIIKILFN KVPNIDKAII
210 220 230 240 250
SVHCHNDLGV ATGNSLSAIL QGARQIECTI NGLGERAGNC ALEEVVMAIK
260 270 280 290 300
TRKDYLKGFY TDIKCENIFK TSKLVSAITN ESIPSHKAIV GSNAFSHSSG
310 320 330 340 350
IHQDGVLKNR QTYEIISPSA IGIHENRMLM TARSGRAMIK TCLENLGYDE
360 370 380 390 400
NTYNLDDVYE RFLRLADKKG QVYDYDLEAL MFLSYENEEE NEFVIEKLSV
410 420 430 440 450
ISGNIPTACV CMRIKEELKT EACTGNGPVE AVFNCIARIT NLKPALKAYS
460 470 480 490 500
INAKSSGVDA QGQVDVDLEF KGRKFHGKGI STDVIEASAQ AFVSAYNAIY
510
RSLKVEERKM A
Length:511
Mass (Da):56,350
Last modified:February 6, 2007 - v1
Checksum:iD446F96FCB66A1D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000538 Genomic DNA. Translation: EAQ71916.1.
RefSeqiYP_001001363.1. NC_008787.1.

Genome annotation databases

EnsemblBacteriaiEAQ71916; EAQ71916; CJJ81176_0017.
KEGGicjj:CJJ81176_0017.
PATRICi20053424. VBICamJej103413_1705.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000538 Genomic DNA. Translation: EAQ71916.1.
RefSeqiYP_001001363.1. NC_008787.1.

3D structure databases

ProteinModelPortaliA1W1X2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi354242.CJJ81176_0017.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiEAQ71916; EAQ71916; CJJ81176_0017.
KEGGicjj:CJJ81176_0017.
PATRICi20053424. VBICamJej103413_1705.

Phylogenomic databases

eggNOGiCOG0119.
HOGENOMiHOG000046859.
KOiK01649.
OMAiWSVHCHN.
OrthoDBiEOG6CGCF3.

Enzyme and pathway databases

UniPathwayiUPA00048; UER00070.
BioCyciCJEJ354242:GC51-1698-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01025. LeuA_type1.
InterProiIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005671. LeuA_bact_synth.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTiSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF110921. SSF110921. 1 hit.
TIGRFAMsiTIGR00973. leuA_bact. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Fouts D.E., Nelson K.E., Sebastian Y.
    Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 81-176.

Entry informationi

Entry nameiLEU1_CAMJJ
AccessioniPrimary (citable) accession number: A1W1X2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 6, 2007
Last modified: April 1, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.