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A1W1T3

- BIOB_CAMJJ

UniProt

A1W1T3 - BIOB_CAMJJ

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Protein

Biotin synthase

Gene

bioB

Organism
Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi16 – 161Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi20 – 201Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi23 – 231Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi60 – 601Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi95 – 951Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi153 – 1531Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCJEJ354242:GC51-1655-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:CJJ81176_1677
OrganismiCampylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176)
Taxonomic identifieri354242 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000000646: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 278278Biotin synthasePRO_0000381287Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi354242.CJJ81176_1677.

Structurei

3D structure databases

ProteinModelPortaliA1W1T3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiTCENTLR.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A1W1T3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQIMLCAISN IASGNCSEDC KYCTQSAHVK TDIQKYRRKE LSQIVLEAKM
60 70 80 90 100
AKKNEALGFC LVTAGLGLDD EKLEYVCEAA KAVQKEVPNL LLIACNGMAS
110 120 130 140 150
VEQLKELKKA GIFSYNHNLE TSKEFFPQIC TTHTWESRFQ TNLNAKEAGL
160 170 180 190 200
MLCCGGIYGM GESEEDRLSF RKSLQELQPF STPINFFIAN ENLKLQVPRL
210 220 230 240 250
NADEALKIVR DTKEALPQSV VMVAGGREVV LRERQYEIFQ AGAGAIVIGD
260 270
YLTTKGEEPS QDIIKLKEMG FTFASECH
Length:278
Mass (Da):30,983
Last modified:February 6, 2007 - v1
Checksum:i837B49BF88D00FCA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000538 Genomic DNA. Translation: EAQ72805.1.
RefSeqiYP_001001324.1. NC_008787.1.

Genome annotation databases

EnsemblBacteriaiEAQ72805; EAQ72805; CJJ81176_1677.
GeneIDi4682612.
KEGGicjj:CJJ81176_1677.
PATRICi20053336. VBICamJej103413_1665.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000538 Genomic DNA. Translation: EAQ72805.1 .
RefSeqi YP_001001324.1. NC_008787.1.

3D structure databases

ProteinModelPortali A1W1T3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 354242.CJJ81176_1677.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai EAQ72805 ; EAQ72805 ; CJJ81176_1677 .
GeneIDi 4682612.
KEGGi cjj:CJJ81176_1677.
PATRICi 20053336. VBICamJej103413_1665.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi TCENTLR.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci CJEJ354242:GC51-1655-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Fouts D.E., Nelson K.E., Sebastian Y.
    Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 81-176.

Entry informationi

Entry nameiBIOB_CAMJJ
AccessioniPrimary (citable) accession number: A1W1T3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 6, 2007
Last modified: November 26, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3