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A1W078 (SYL_CAMJJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:CJJ81176_1109
OrganismCampylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) [Complete proteome] [HAMAP]
Taxonomic identifier354242 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter

Protein attributes

Sequence length809 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 809809Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009318

Regions

Motif40 – 5011"HIGH" region HAMAP-Rule MF_00049
Motif579 – 5835"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5821ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A1W078 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: C990B140335DCC73

FASTA80993,356
        10         20         30         40         50         60 
MAYEASLIEK KWQKIWDENE YFEPKDDLNL PKKYILSMFP YPSGRIHMGH VRNYTIGDAL 

        70         80         90        100        110        120 
ARYYRKIGFN VLHPIGFDSF GMPAENAAIK HKIHPKSWTY ENIAYMKKEL FSLGFSFSKK 

       130        140        150        160        170        180 
RMLATSDPLY TKFEQEFFIK MFEKGLIYTK EANVNWCEQD QTVLANEQVE DGKCWRCGHE 

       190        200        210        220        230        240 
VAQKKMPGYY VKITAYAEEL LKDLEELKDK WPNQVLTMQE NWIGKSEGLE FSLNLDEESK 

       250        260        270        280        290        300 
QKTKESSLEV FTTRADTIYG VSYIALAPEH KIVQNLLSQN LLNQDVLNKI KAIQNQSPRE 

       310        320        330        340        350        360 
RQSSEKEGYF LGIYAIHPLS GEKIPLWVAN FILADYGSGA VMAVPAHDER DFEFATKYNL 

       370        380        390        400        410        420 
AIKQVIQTQE NLPYTQKSGK LIHSQEFDNL DCNEARLKII SQFEAKNIGK RVVNFKIRDW 

       430        440        450        460        470        480 
GVSRQRYWGA PIPMIKCQSC GIVPQKLENL PITLPEDVQI TGEGNPLDKH PTWKNCICPK 

       490        500        510        520        530        540 
CGKEAQKESD TLDTFFESSW YFARFASDEK TWQEKALDEK SVKYWMSVDQ YIGGIEHAIL 

       550        560        570        580        590        600 
HLLYARFFQK ALRDLGYLTQ NEPFDRLLTQ GMVLKDGAKM SKSKGNVVDP DEIIEKYGAD 

       610        620        630        640        650        660 
TARLFILFAA PPAKELEWND DAVEGAYRFI CKLYDRAQNV KKGELVELKQ ENLNKEEKYA 

       670        680        690        700        710        720 
RLKVYEALKK SFEVYHQSFA FNTLIAACME ALNALALCKN EALEQEAFYI ILNILEPIIP 

       730        740        750        760        770        780 
HVCFELSEEL FKCKNFKKLE LKEEVFVKDT LNLAVSINGK KRAEFEISSS ASKEEILAFA 

       790        800 
KENTAKWLEG KSIVKEIYVE GKLVNLVIK 

« Hide

References

[1]Fouts D.E., Nelson K.E., Sebastian Y.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 81-176.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000538 Genomic DNA. Translation: EAQ72112.1.
RefSeqYP_001000769.1. NC_008787.1.

3D structure databases

ProteinModelPortalA1W078.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING354242.CJJ81176_1109.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEAQ72112; EAQ72112; CJJ81176_1109.
GeneID4683724.
KEGGcjj:CJJ81176_1109.
PATRIC20052191. VBICamJej103413_1108.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycCJEJ354242:GC51-1085-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_CAMJJ
AccessionPrimary (citable) accession number: A1W078
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: April 16, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries