A1VZF8 (NADE_CAMJJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	CJJ81176_0831

Organism

Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) [Complete proteome] [HAMAP]

Taxonomic identifier

354242 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000077542
Regions
	Nucleotide binding	29 – 36	8	ATP By similarity
Sites
	Active site	31	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1VZF8 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: A92C989F968078FB	FASTA	246	27,433
	10 20 30 40 50 60 MDWQKITEKM CDFIQEKVKN SQSQGVVLGL SGGIDSALVA TLCKRALKEN VFALLMPTQI 70 80 90 100 110 120 SNKANLEDAL RLCADLNLEY KIIEIQSILD AFIKQSENTT LVSLGNFAAR IRMSLLYDYS 130 140 150 160 170 180 ALKNSLVIGT SNKSELLLGY GTIYGDLACA FNPIGSLYKS EIYALAKYLN LHENFIKKAP 190 200 210 220 230 240 SADLWENQSD EADLGFSYAK IDEGLKALET NDEKLLRTLD PSLIAMLKNR MQKNAFKGKM PEILEI

References

[1]	Fouts D.E., Nelson K.E., Sebastian Y. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 81-176.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000538 Genomic DNA. Translation: EAQ72323.1.
RefSeq	YP_001000499.1. NC_008787.1.
3D structure databases
ProteinModelPortal	A1VZF8.
ModBase	Search...
Protein-protein interaction databases
STRING	A1VZF8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4683760.
GenomeReviews	Gene locus CJJ81176_0831 in contig CP000538_GR.
KEGG	cjj:CJJ81176_0831.
PATRIC	20051623. VBICamJej103413_0835.
TIGR	CJJ81176_0831.
Phylogenomic databases
eggNOG	COG0171.
HOGENOM	HBG351567.
OMA	YDISARD.
PhylomeDB	A1VZF8.
ProtClustDB	PRK13980.
Enzyme and pathway databases
BioCyc	CJEJ354242:CJJ81176_0831-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_CAMJJ

Accession

Primary (citable) accession number: A1VZF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program