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Protein

D-alanine--D-alanine ligase

Gene

ddl

Organism
Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cell wall formation.UniRule annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathway:ipeptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi284 – 2841Magnesium or manganese 1UniRule annotation
Metal bindingi296 – 2961Magnesium or manganese 1UniRule annotation
Metal bindingi296 – 2961Magnesium or manganese 2UniRule annotation
Metal bindingi298 – 2981Magnesium or manganese 2UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi159 – 21153ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCJEJ354242:GC51-792-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseUniRule annotation (EC:6.3.2.4UniRule annotation)
Alternative name(s):
D-Ala-D-Ala ligaseUniRule annotation
D-alanylalanine synthetaseUniRule annotation
Gene namesi
Name:ddlUniRule annotation
Ordered Locus Names:CJJ81176_0819
OrganismiCampylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176)
Taxonomic identifieri354242 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000000646 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 346346D-alanine--D-alanine ligasePRO_1000074767Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi354242.Cjejjejuni_010100004220.

Structurei

3D structure databases

ProteinModelPortaliA1VZE6.
SMRiA1VZE6. Positions 1-323.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini133 – 327195ATP-graspUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1181.
HOGENOMiHOG000102494.
KOiK01921.
OMAiIPYVGCD.
OrthoDBiEOG6ND0KB.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPiMF_00047. Dala_Dala_lig.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR23132. PTHR23132. 1 hit.
PfamiPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1VZE6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFAILFGGN SYEHEISIVS AVVLKKVINQ NLEFIFCDEE RRFYHIPSEK
60 70 80 90 100
MNSKTFSTKA YKKEKELFIK QGGFFSKGFL KENKLECECV INLIHGRDGE
110 120 130 140 150
DGKIAALFEF YSIKFIGPRL EASVLSFNKE LTKLYAKSVG VKTLDYTMVR
160 170 180 190 200
KGQNSKEKLS FPCIIKPARL GSSIGISIVK DEKDLEYAKD VGFEFDNDLV
210 220 230 240 250
VEEFKNNIKE YNLAGCMIND EFVFSIIEEP KKKEFLDFEQ KYLSFSGHNE
260 270 280 290 300
LIEANLSEEL KEKLKDSFKK IYNPLFKGAL IRCDFFILDN EIYLNEINPN
310 320 330 340
PGSLANYLFK DFSTTLNALA DQISLEKMIK ISYNFLHSIN GQKGKL
Length:346
Mass (Da):39,800
Last modified:February 6, 2007 - v1
Checksum:iCDFAF5AB2726749A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000538 Genomic DNA. Translation: EAQ72375.1.
RefSeqiWP_002869413.1. NZ_AASL01000001.1.

Genome annotation databases

EnsemblBacteriaiEAQ72375; EAQ72375; CJJ81176_0819.
KEGGicjj:CJJ81176_0819.
PATRICi20051599. VBICamJej103413_0823.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000538 Genomic DNA. Translation: EAQ72375.1.
RefSeqiWP_002869413.1. NZ_AASL01000001.1.

3D structure databases

ProteinModelPortaliA1VZE6.
SMRiA1VZE6. Positions 1-323.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi354242.Cjejjejuni_010100004220.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiEAQ72375; EAQ72375; CJJ81176_0819.
KEGGicjj:CJJ81176_0819.
PATRICi20051599. VBICamJej103413_0823.

Phylogenomic databases

eggNOGiCOG1181.
HOGENOMiHOG000102494.
KOiK01921.
OMAiIPYVGCD.
OrthoDBiEOG6ND0KB.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciCJEJ354242:GC51-792-MONOMER.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPiMF_00047. Dala_Dala_lig.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR23132. PTHR23132. 1 hit.
PfamiPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Fouts D.E., Nelson K.E., Sebastian Y.
    Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 81-176.

Entry informationi

Entry nameiDDL_CAMJJ
AccessioniPrimary (citable) accession number: A1VZE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 6, 2007
Last modified: July 22, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.