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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi41Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi44Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi48Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi76Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei78Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei146Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei171Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei175Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei416Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei420Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei526Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei574Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei601Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei889Substrate; via amide nitrogenUniRule annotation1
Binding sitei897Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei914Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:CJJ81176_0801
OrganismiCampylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176)
Taxonomic identifieri354242 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000000646 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Tat-type signalUniRule annotationAdd BLAST30
ChainiPRO_100006971431 – 923Periplasmic nitrate reductaseUniRule annotationAdd BLAST893

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Protein-protein interaction databases

STRINGi354242.Cjejjejuni_010100004115

Structurei

3D structure databases

ProteinModelPortaliA1VZC8
SMRiA1VZC8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 904Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni208 – 215Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni551 – 552Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni813 – 822Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW Bacteria
COG0243 LUCA
HOGENOMiHOG000031441
KOiK02567
OMAiTQHWRQQ

Family and domain databases

HAMAPiMF_01630 Nitrate_reduct_NapA, 1 hit
InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR027467 MopterinOxRdtase_cofactor_BS
IPR010051 Periplasm_NO3_reductase_lsu
IPR019546 TAT_signal_bac_arc
PANTHERiPTHR11615:SF123 PTHR11615:SF123, 2 hits
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR01706 NAPA, 1 hit
TIGR01409 TAT_signal_seq, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1VZC8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRRDFIKNT AIASAASVAG LSVPSSMLGA QEDWKWDKAV CRFCGTGCGI
60 70 80 90 100
MIARKDGKIV ATKGDPAAPV NRGLNCIKGY FNAKIMYGED RLVMPLLRMN
110 120 130 140 150
EKGEFDKKGK FQQVSWQRAF DEMEKQFKKA YNELGVTGIG IFGSGQYTIQ
160 170 180 190 200
EGYAALKLAK AGFRTNNIDP NARHCMASAV VGFMQTFGVD EPSGCYDDIE
210 220 230 240 250
LTDTIITWGA NMAEMHPILW SRVSDRKLSN LDKVKVVNLS TFSNRTSNIA
260 270 280 290 300
DIEIIFKPNT DLAIWNYIAR EIVYNHPEAM DMKFIKDHCV FATGYADIGY
310 320 330 340 350
GMRNNPNHPK FKESEKDTVE KENVITLDDE EAASLSYLGV KAGDKFEMKH
360 370 380 390 400
QGVADKNWEI SFEEFKKGLA PYTLEYTAKV AKGDDNESLE DFKKKLQELA
410 420 430 440 450
NLYIEKNRKV VSFWTMGFNQ HTRGSWVNEQ AYMVHFLLGK QAKPGSGAFS
460 470 480 490 500
LTGQPSACGT AREVGTFSHR LPADMVVANP KHREISEKIW KVPAKTINPK
510 520 530 540 550
PGSPYLNIMR DLEDGKIKFA WVQVNNPWQN TANANHWIAA AREMDNFIVV
560 570 580 590 600
SDCYPGISAK VADLILPSAM IYEKWGAYGN AERRTQHWKQ QVLPVGAAMS
610 620 630 640 650
DTWQILEFAK RFKLKEVWKE QKVDNKLTLP SVLEEAKAMG YSEDDTLFDV
660 670 680 690 700
LFANKEAKSF NPNDAIAKGF DNTDVKGDER KIQGSDGKEF TGYGFFVQKY
710 720 730 740 750
LWEEYRKFGL GHGHDLADFD TYHKVRGLRW PVVNGKETQW RFNTKFDYYA
760 770 780 790 800
KKAAPNSDFA FYGDFNKMLT NGDLIAPKDE KEHSIKNKAK IFFRPFMKAP
810 820 830 840 850
ERPSKEYPFW LATGRVLEHW HSGTMTMRVP ELYRAVPEAL CYMSEKDGEK
860 870 880 890 900
LGLNQGDLVW VESRRGKVKA RVDMRGRNKP PVGLVYVPWF DENVYINKVT
910 920
LDATCPLSKQ TDFKKCAVKI YKA
Length:923
Mass (Da):104,771
Last modified:February 6, 2007 - v1
Checksum:i43A93C661081EE05
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000538 Genomic DNA Translation: EAQ72370.1
RefSeqiWP_002869179.1, NC_008787.1

Genome annotation databases

EnsemblBacteriaiEAQ72370; EAQ72370; CJJ81176_0801
KEGGicjj:CJJ81176_0801

Similar proteinsi

Entry informationi

Entry nameiNAPA_CAMJJ
AccessioniPrimary (citable) accession number: A1VZC8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 6, 2007
Last modified: March 28, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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