Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A1VZC8

- NAPA_CAMJJ

UniProt

A1VZC8 - NAPA_CAMJJ

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (06 Feb 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi41 – 411Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi44 – 441Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi48 – 481Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi76 – 761Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciCJEJ354242:GC51-774-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:CJJ81176_0801
    OrganismiCampylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176)
    Taxonomic identifieri354242 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
    ProteomesiUP000000646: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3030Tat-type signalUniRule annotationAdd
    BLAST
    Chaini31 – 923893Periplasmic nitrate reductasePRO_1000069714Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi354242.CJJ81176_0801.

    Structurei

    3D structure databases

    ProteinModelPortaliA1VZC8.
    SMRiA1VZC8. Positions 32-922.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini34 – 90574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiFNQHQRG.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A1VZC8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNRRDFIKNT AIASAASVAG LSVPSSMLGA QEDWKWDKAV CRFCGTGCGI    50
    MIARKDGKIV ATKGDPAAPV NRGLNCIKGY FNAKIMYGED RLVMPLLRMN 100
    EKGEFDKKGK FQQVSWQRAF DEMEKQFKKA YNELGVTGIG IFGSGQYTIQ 150
    EGYAALKLAK AGFRTNNIDP NARHCMASAV VGFMQTFGVD EPSGCYDDIE 200
    LTDTIITWGA NMAEMHPILW SRVSDRKLSN LDKVKVVNLS TFSNRTSNIA 250
    DIEIIFKPNT DLAIWNYIAR EIVYNHPEAM DMKFIKDHCV FATGYADIGY 300
    GMRNNPNHPK FKESEKDTVE KENVITLDDE EAASLSYLGV KAGDKFEMKH 350
    QGVADKNWEI SFEEFKKGLA PYTLEYTAKV AKGDDNESLE DFKKKLQELA 400
    NLYIEKNRKV VSFWTMGFNQ HTRGSWVNEQ AYMVHFLLGK QAKPGSGAFS 450
    LTGQPSACGT AREVGTFSHR LPADMVVANP KHREISEKIW KVPAKTINPK 500
    PGSPYLNIMR DLEDGKIKFA WVQVNNPWQN TANANHWIAA AREMDNFIVV 550
    SDCYPGISAK VADLILPSAM IYEKWGAYGN AERRTQHWKQ QVLPVGAAMS 600
    DTWQILEFAK RFKLKEVWKE QKVDNKLTLP SVLEEAKAMG YSEDDTLFDV 650
    LFANKEAKSF NPNDAIAKGF DNTDVKGDER KIQGSDGKEF TGYGFFVQKY 700
    LWEEYRKFGL GHGHDLADFD TYHKVRGLRW PVVNGKETQW RFNTKFDYYA 750
    KKAAPNSDFA FYGDFNKMLT NGDLIAPKDE KEHSIKNKAK IFFRPFMKAP 800
    ERPSKEYPFW LATGRVLEHW HSGTMTMRVP ELYRAVPEAL CYMSEKDGEK 850
    LGLNQGDLVW VESRRGKVKA RVDMRGRNKP PVGLVYVPWF DENVYINKVT 900
    LDATCPLSKQ TDFKKCAVKI YKA 923
    Length:923
    Mass (Da):104,771
    Last modified:February 6, 2007 - v1
    Checksum:i43A93C661081EE05
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000538 Genomic DNA. Translation: EAQ72370.1.
    RefSeqiYP_001000469.1. NC_008787.1.

    Genome annotation databases

    EnsemblBacteriaiEAQ72370; EAQ72370; CJJ81176_0801.
    GeneIDi4683033.
    KEGGicjj:CJJ81176_0801.
    PATRICi20051563. VBICamJej103413_0805.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000538 Genomic DNA. Translation: EAQ72370.1 .
    RefSeqi YP_001000469.1. NC_008787.1.

    3D structure databases

    ProteinModelPortali A1VZC8.
    SMRi A1VZC8. Positions 32-922.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 354242.CJJ81176_0801.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai EAQ72370 ; EAQ72370 ; CJJ81176_0801 .
    GeneIDi 4683033.
    KEGGi cjj:CJJ81176_0801.
    PATRICi 20051563. VBICamJej103413_0805.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi FNQHQRG.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci CJEJ354242:GC51-774-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Fouts D.E., Nelson K.E., Sebastian Y.
      Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 81-176.

    Entry informationi

    Entry nameiNAPA_CAMJJ
    AccessioniPrimary (citable) accession number: A1VZC8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: February 6, 2007
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3