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A1VZC8 (NAPA_CAMJJ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:CJJ81176_0801
OrganismCampylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) [Complete proteome] [HAMAP]
Taxonomic identifier354242 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter

Protein attributes

Sequence length923 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030Tat-type signal Potential
Chain31 – 923893Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_1000069714

Regions

Domain34 – 90574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding411Iron-sulfur (4Fe-4S) By similarity
Metal binding441Iron-sulfur (4Fe-4S) By similarity
Metal binding481Iron-sulfur (4Fe-4S) By similarity
Metal binding761Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A1VZC8 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 43A93C661081EE05

FASTA923104,771
        10         20         30         40         50         60 
MNRRDFIKNT AIASAASVAG LSVPSSMLGA QEDWKWDKAV CRFCGTGCGI MIARKDGKIV 

        70         80         90        100        110        120 
ATKGDPAAPV NRGLNCIKGY FNAKIMYGED RLVMPLLRMN EKGEFDKKGK FQQVSWQRAF 

       130        140        150        160        170        180 
DEMEKQFKKA YNELGVTGIG IFGSGQYTIQ EGYAALKLAK AGFRTNNIDP NARHCMASAV 

       190        200        210        220        230        240 
VGFMQTFGVD EPSGCYDDIE LTDTIITWGA NMAEMHPILW SRVSDRKLSN LDKVKVVNLS 

       250        260        270        280        290        300 
TFSNRTSNIA DIEIIFKPNT DLAIWNYIAR EIVYNHPEAM DMKFIKDHCV FATGYADIGY 

       310        320        330        340        350        360 
GMRNNPNHPK FKESEKDTVE KENVITLDDE EAASLSYLGV KAGDKFEMKH QGVADKNWEI 

       370        380        390        400        410        420 
SFEEFKKGLA PYTLEYTAKV AKGDDNESLE DFKKKLQELA NLYIEKNRKV VSFWTMGFNQ 

       430        440        450        460        470        480 
HTRGSWVNEQ AYMVHFLLGK QAKPGSGAFS LTGQPSACGT AREVGTFSHR LPADMVVANP 

       490        500        510        520        530        540 
KHREISEKIW KVPAKTINPK PGSPYLNIMR DLEDGKIKFA WVQVNNPWQN TANANHWIAA 

       550        560        570        580        590        600 
AREMDNFIVV SDCYPGISAK VADLILPSAM IYEKWGAYGN AERRTQHWKQ QVLPVGAAMS 

       610        620        630        640        650        660 
DTWQILEFAK RFKLKEVWKE QKVDNKLTLP SVLEEAKAMG YSEDDTLFDV LFANKEAKSF 

       670        680        690        700        710        720 
NPNDAIAKGF DNTDVKGDER KIQGSDGKEF TGYGFFVQKY LWEEYRKFGL GHGHDLADFD 

       730        740        750        760        770        780 
TYHKVRGLRW PVVNGKETQW RFNTKFDYYA KKAAPNSDFA FYGDFNKMLT NGDLIAPKDE 

       790        800        810        820        830        840 
KEHSIKNKAK IFFRPFMKAP ERPSKEYPFW LATGRVLEHW HSGTMTMRVP ELYRAVPEAL 

       850        860        870        880        890        900 
CYMSEKDGEK LGLNQGDLVW VESRRGKVKA RVDMRGRNKP PVGLVYVPWF DENVYINKVT 

       910        920 
LDATCPLSKQ TDFKKCAVKI YKA 

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References

[1]Fouts D.E., Nelson K.E., Sebastian Y.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 81-176.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000538 Genomic DNA. Translation: EAQ72370.1.
RefSeqYP_001000469.1. NC_008787.1.

3D structure databases

ProteinModelPortalA1VZC8.
SMRA1VZC8. Positions 32-922.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING354242.CJJ81176_0801.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEAQ72370; EAQ72370; CJJ81176_0801.
GeneID4683033.
KEGGcjj:CJJ81176_0801.
PATRIC20051563. VBICamJej103413_0805.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAFNQHQRG.
OrthoDBEOG6CVV7G.
ProtClustDBPRK13532.

Enzyme and pathway databases

BioCycCJEJ354242:GC51-774-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_CAMJJ
AccessionPrimary (citable) accession number: A1VZC8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 6, 2007
Last modified: February 19, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families