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A1VTV4 (SYL_POLNA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Pnap_3786
OrganismPolaromonas naphthalenivorans (strain CJ2) [Complete proteome] [HAMAP]
Taxonomic identifier365044 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaePolaromonas

Protein attributes

Sequence length888 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 888888Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009390

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif640 – 6445"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6431ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A1VTV4 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: FB63D4C5260D8D59

FASTA88898,298
        10         20         30         40         50         60 
MQDKYNHLDV ERSAQTHWNA ADAYRVTEDA SRKKYYACSM LPYPSGKLHM GHVRNYTIND 

        70         80         90        100        110        120 
MLSRYLRMKG YNVLMPMGWD AFGLPAENAA LKNGVPPAQW TYDNIAYMKK QMQAMGLAVD 

       130        140        150        160        170        180 
WSREIATCDP SYYKWNQWLF LKMLEKGIAY RKTQVVNWDP VDQTVLANEQ VIDGKGWRTG 

       190        200        210        220        230        240 
ATVEKREIPG YYLKITDYAE ELLGSVQHKL PGWPERVKLM QENWIGKSEG VRFAFLHDIK 

       250        260        270        280        290        300 
DASGALIGDG QMYVFTTRAD TIMGVTFCAV APEHPLAVHA AASNPALAAF VEECKSGGTT 

       310        320        330        340        350        360 
EAELATQEKK GQPTGLFVTH PLTGDKVEVW VGNYVLMSYG DGAVMGVPAH DERDFEFAKK 

       370        380        390        400        410        420 
YGLPIKQVTD VKGQAYSLDA WADWYGDKQH GIAINSGKYD GLAFKAAVDA IAADLAALGL 

       430        440        450        460        470        480 
GEKKTTWRLR DWGVSRQRYW GTPIPIIHCD EHGAVPVPEK DLPVVLPMDC IPDGSGNPLH 

       490        500        510        520        530        540 
KHEGFHAGVV CPVCGKPARR ETDTMDTFVD SSWYFMRYCD PKNSEAMVAG GADYWMPMDQ 

       550        560        570        580        590        600 
YIGGIEHAIL HLLYARFWTK VMRDLGLVKV DEPFTKLLTQ GMVLNHIYSR RTDKGGKEYF 

       610        620        630        640        650        660 
WPHDVEHVLD DTGKIAGARL KNAVGDLPVG TAIDYEGVGT MSKSKNNGVD PQDIIEKYGA 

       670        680        690        700        710        720 
DTARLYTMFT APPEATLEWN DAGVEGSYRF LRRVWNFGVK LNAMNSGANG ADAACAKGLF 

       730        740        750        760        770        780 
DKETKALRLE VHTLLKQVDY DYQRMQYNTV VSGGMKLLNA LEDFKGEISA ASLAALREGF 

       790        800        810        820        830        840 
SVLLRCLYPA APHLTHALWS ELGYAAEAGD LLDTPWPEVD ASALQQDEIE LMLQINGKLR 

       850        860        870        880 
GSVTVPAGAD KAVIEAAALA SEAFVKQAAG APAKKVIVVP GRLVNIVV 

« Hide

References

[1]"Complete sequence of chromosome 1 of Polaromonas naphthalenivorans CJ2."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D.R., Brettin T., Bruce D., Han C., Tapia R., Brainard J., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Madsen E.L., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CJ2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000529 Genomic DNA. Translation: ABM39082.1.
RefSeqYP_984003.1. NC_008781.1.

3D structure databases

ProteinModelPortalA1VTV4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING365044.Pnap_3786.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM39082; ABM39082; Pnap_3786.
GeneID4686290.
KEGGpna:Pnap_3786.
PATRIC22952337. VBIPolNap76733_4684.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycPNAP365044:GJ8X-3841-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_POLNA
AccessionPrimary (citable) accession number: A1VTV4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: April 16, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries