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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Polaromonas naphthalenivorans (strain CJ2)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei75S-adenosyl-L-methionineUniRule annotation1
Binding sitei100S-adenosyl-L-methionineUniRule annotation1
Binding sitei127S-adenosyl-L-methionineUniRule annotation1
Active sitei150By similarity1
Binding sitei150S-adenosyl-L-methionineUniRule annotation1
Binding sitei154SubstrateUniRule annotation1
Binding sitei186SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Pnap_2936
OrganismiPolaromonas naphthalenivorans (strain CJ2)
Taxonomic identifieri365044 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaePolaromonas
Proteomesi
  • UP000000644 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002881961 – 246tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST246

Interactioni

Protein-protein interaction databases

STRINGi365044.Pnap_2936.

Structurei

3D structure databases

ProteinModelPortaliA1VRF7.
SMRiA1VRF7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni156 – 161Interaction with RNAUniRule annotation6
Regioni225 – 228Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000073968.
KOiK03439.
OMAiPDPWHKS.
OrthoDBiPOG091H015P.

Family and domain databases

HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiView protein in InterPro
IPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
PANTHERiPTHR23417. PTHR23417. 1 hit.
PfamiView protein in Pfam
PF02390. Methyltransf_4. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiView protein in PROSITE
PS51625. SAM_MT_TRMB. 1 hit.

Sequencei

Sequence statusi: Complete.

A1VRF7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIESSSPTPP ALHEGAPADV SHPRNIRSFV RRTGRTTVGQ AKAFADVGPR
60 70 80 90 100
FLLAYSGLPM DFSAVYGRDA PTILEIGFGM GEATAHIAGL MPEKNFLCCE
110 120 130 140 150
VHTPGVGALL KRIDEQALTN IRILQHDAVE VIDHMLPLGS LDGAHIFFPD
160 170 180 190 200
PWHKTKHNKR RLIQAPLIAK LAARLKPGGY LHCATDWQPY AEQILEVLSA
210 220 230 240
EPLLKNTADA ASDGYAPKPG YRPLTKFENR GIKLGHGVWD VVFTRA
Length:246
Mass (Da):26,899
Last modified:February 6, 2007 - v1
Checksum:i78D01252E4BADB7B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000529 Genomic DNA. Translation: ABM38235.1.
RefSeqiWP_011802309.1. NC_008781.1.

Genome annotation databases

EnsemblBacteriaiABM38235; ABM38235; Pnap_2936.
KEGGipna:Pnap_2936.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiTRMB_POLNA
AccessioniPrimary (citable) accession number: A1VRF7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: February 6, 2007
Last modified: June 7, 2017
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families