A1VQC4 (A1VQC4_POLNA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108 RuleBase RU003490
EC=2.7.7.60 HAMAP MF_00108 RuleBase RU003490

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase HAMAP MF_00108
MEP cytidylyltransferase HAMAP MF_00108

Gene names

Name:	ispD HAMAP MF_00108
Ordered Locus Names:	Pnap_2549

Organism

Polaromonas naphthalenivorans (strain CJ2) [Complete proteome] [HAMAP]

Taxonomic identifier

365044 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Polaromonas

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108 RuleBase RU003490 SAAS SAAS018294
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108 RuleBase RU003490 SAAS SAAS018294
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108 SAAS SAAS018294 RuleBase RU004138
Sequence similarities	Belongs to the IspD family. HAMAP MF_00108 RuleBase RU004393

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00108 SAAS SAAS018294 RuleBase RU004393
Molecular function	Nucleotidyltransferase HAMAP MF_00108 SAAS SAAS018294 RuleBase RU004393 EMBL ABM37852.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Site	25	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	34	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	165	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108
	Site	221	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108

Sequences

	Sequence		Length	Mass (Da)
	A1VQC4 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 5B05283C4C7D8128	FASTA	242	25,468
	10 20 30 40 50 60 MTNSADTRSR SRFFALIPCA GQGSRAGVVA GQAKQYQKIA GQAMVLHTLA AFLAVGRLAR 70 80 90 100 110 120 TLVVVAPGDG FFDSITEKQF DVAACGGASR ATSVRRGLDA LLQSGAQAGD WVLVHDAARC 130 140 150 160 170 180 LVTPGQIKQL IDACRHDEVG GLLAHQLPDT LKQAASGRVS GTLDRSDKWL AQTPQMFRIG 190 200 210 220 230 240 LLTQALDAAE SAGRAVTDES SAIEALGLAP RLVPGSAQNF KVTYPDDFAL AEAILLSRVR PD

References

[1]

"Complete sequence of chromosome 1 of Polaromonas naphthalenivorans CJ2." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D.R., Brettin T., Bruce D., Han C., Tapia R., Brainard J., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Madsen E.L., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CJ2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000529 Genomic DNA. Translation: ABM37852.1.
RefSeq	YP_982773.1. NC_008781.1.
3D structure databases
ProteinModelPortal	A1VQC4.
ModBase	Search...
Protein-protein interaction databases
STRING	A1VQC4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4686630.
GenomeReviews	Gene locus Pnap_2549 in contig CP000529_GR.
KEGG	pna:Pnap_2549.
PATRIC	22949769. VBIPolNap76733_3417.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1211.
HOGENOM	HBG672839.
OMA	SKVIVVC.
PhylomeDB	A1VQC4.
ProtClustDB	CLSK723488.
Enzyme and pathway databases
BioCyc	PNAP365044:PNAP_2549-MONOMER.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A1VQC4_POLNA

Accession

Primary (citable) accession number: A1VQC4

Entry history

Integrated into UniProtKB/TrEMBL:	February 6, 2007
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)