Reviewed, UniProtKB/Swiss-Prot A1VNR3 (F16A2_POLNA)

Names and origin

Protein names

Recommended name:
    Fructose-1,6-bisphosphatase class 1 2
      Short name=FBPase class 1 2
    EC=3.1.3.11
Alternative name(s):
    D-fructose-1,6-bisphosphate 1-phosphohydrolase class 1 2

Gene names

Name:	fbp2
Ordered Locus Names:	Pnap_1982

Organism

Polaromonas naphthalenivorans (strain CJ2) [Complete proteome] [HAMAP]

Taxonomic identifier

365044 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Polaromonas

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate. HAMAP MF_01855
Cofactor	Binds 2 magnesium ions per subunit By similarity. HAMAP MF_01855
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_01855
Subunit structure	Homotetramer By similarity. HAMAP MF_01855
Subcellular location	Cytoplasm Potential HAMAP MF_01855.
Sequence similarities	Belongs to the FBPase class 1 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fructose 1,6-bisphosphate 1-phosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Fructose-1,6-bisphosphatase class 1 2 HAMAP MF_01855		PRO_0000364629
Regions
	Region	124 – 127	4	Substrate binding By similarity
Sites
	Metal binding	99	1	Magnesium 1 By similarity
	Metal binding	121	1	Magnesium 1 By similarity
	Metal binding	121	1	Magnesium 2 By similarity
	Metal binding	123	1	Magnesium 1; via carbonyl oxygen By similarity
	Metal binding	124	1	Magnesium 2 By similarity
	Metal binding	292	1	Magnesium 2 By similarity
	Binding site	220	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1VNR3-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 97599F184FFF6B28	FASTA	364	40,284
	10 20 30 40 50 60 MPLNKRFTLT QYLIQERRRF PDARGDFNAL ILDVALACKA IARAVAFGEL GGMLGNHDAD 70 80 90 100 110 120 AGGSINVQGE TQKKLDVISN QYFTRMNEWG GHLAGMASEE MDDAYQIPAE HPRGKYLLVF 130 140 150 160 170 180 DPLDGSSNID VNVSVGSIFS ILRAPQEAVE SGRDVVEADF FQPGAEQVAA GYALYGPTTM 190 200 210 220 230 240 LVLSVGNGVA GFTLDPMLGE FMLTHDKLQV PENTQEFAIN ASNSRFWEPP VKRYVDECLA 250 260 270 280 290 300 GKTGPRDKDF NMRWIASMVA EAHRILMRGG VFLYPRDSKD AAKPGRLRLL YEANPIGFIM 310 320 330 340 350 360 EQAGGRASTG REPMLGVQPT SLHQRIGLIF GSKNEVERIE RYHAEPARAE MHNPLFAERS LFRS

References

[1]

"Complete sequence of chromosome 1 of Polaromonas naphthalenivorans CJ2." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D.R., Brettin T., Bruce D., Han C., Tapia R., Brainard J., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Madsen E.L., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000529 Genomic DNA. Translation: ABM37291.1.
RefSeq	YP_982212.1.
3D structure databases
SMR	A1VNR3. Positions 7-344.
ModBase	Search...
Protein-protein interaction databases
STRING	A1VNR3.
Genome annotation databases
GeneID	4688089.
GenomeReviews	Gene locus Pnap_1982 in contig CP000529_GR.
KEGG	pna:Pnap_1982.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0158.
HOGENOM	HBG731261.
OMA	DGDGQKA.
PhylomeDB	A1VNR3.
Family and domain databases
HAMAP	MF_01855. FBPase_class1. [Tree]
InterPro	IPR000146. Fructose_bisphosphatase. IPR020548. Fructose_bisphosphatase_AS. [Graphical view]
PANTHER	PTHR11556. In_FB_phphtase. 1 hit.
Pfam	PF00316. FBPase. 1 hit. [Graphical view]
PRINTS	PR00115. F16BPHPHTASE.
PROSITE	PS00124. FBPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F16A2_POLNA

Accession

Primary (citable) accession number: A1VNR3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	February 6, 2007
Last modified:	January 19, 2010
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)