A1VN50 (LPXD_POLNA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	Pnap_1768

Organism

Polaromonas naphthalenivorans (strain CJ2) [Complete proteome] [HAMAP]

Taxonomic identifier

365044 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Polaromonas

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_1000050948
Sites
	Active site	246	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1VN50 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 78780937CF317CA1	FASTA	355	37,114
	10 20 30 40 50 60 MTLRLADMTE LLADKVQAEL LGNSELLIER LSTLEAAGPN DLAFLSHPKY LGQLAQSAAG 70 80 90 100 110 120 CVIVAPSARE AALKRGPCIV VDDPYYYFAL VTQLWKRQHF PGAAPAIHAS ACIDPAAIIS 130 140 150 160 170 180 PHVSIGAFAC IAAGAVIGEG ARIAEHCVIG ANAIVGANSR LSARVTVADD CRIGERCIIH 190 200 210 220 230 240 PGAVIGADGF GFAPHDGQWV KIEQLGAVRI GNDVEIGANT CIDRGALQDT VIEDGVKLDN 250 260 270 280 290 300 LVQIAHNVRV GRHSAMAGCA GVAGSATIGA HCTVGGGAIV LGHLRLADGV HVSAASIVTR 310 320 330 340 350 SLLKPGHYTG LFPIDDNAAW EKNAATLKQL HALRERLKQT EKSLLQLQGS LEEKP

References

[1]

"Complete sequence of chromosome 1 of Polaromonas naphthalenivorans CJ2." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D.R., Brettin T., Bruce D., Han C., Tapia R., Brainard J., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Madsen E.L., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CJ2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000529 Genomic DNA. Translation: ABM37078.1.
RefSeq	YP_981999.1. NC_008781.1.
3D structure databases
ProteinModelPortal	A1VN50.
ModBase	Search...
Protein-protein interaction databases
STRING	A1VN50.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4687777.
GenomeReviews	Gene locus Pnap_1768 in contig CP000529_GR.
KEGG	pna:Pnap_1768.
PATRIC	22948170. VBIPolNap76733_2627.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1044.
HOGENOM	HBG469615.
OMA	CFVGKNT.
PhylomeDB	A1VN50.
ProtClustDB	PRK00892.
Enzyme and pathway databases
BioCyc	PNAP365044:PNAP_1768-MONOMER.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 5 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_POLNA

Accession

Primary (citable) accession number: A1VN50

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program