A1VN15 (HEM6_POLNA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Coproporphyrinogen-III oxidase, aerobic
Short name=Coprogen oxidase
Short name=Coproporphyrinogenase
EC=1.3.3.3

Gene names

Name:	hemF
Ordered Locus Names:	Pnap_1730

Organism

Polaromonas naphthalenivorans (strain CJ2) [Complete proteome] [HAMAP]

Taxonomic identifier

365044 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Polaromonas

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III By similarity. HAMAP MF_00333
Catalytic activity	Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O. HAMAP MF_00333
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1. HAMAP MF_00333
Subunit structure	Homodimer By similarity. HAMAP MF_00333
Subcellular location	Cytoplasm By similarity HAMAP MF_00333.
Sequence similarities	Belongs to the aerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	porphyrin-containing compound biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: EC protein homodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Coproporphyrinogen-III oxidase, aerobic HAMAP MF_00333		PRO_1000019474
Regions
	Region	53 – 62	10	Important for dimerization By similarity
	Region	113 – 115	3	Substrate binding By similarity
	Region	247 – 282	36	Important for dimerization By similarity
	Region	265 – 270	6	Substrate binding By similarity
Sites
	Active site	111	1	Proton donor By similarity
	Binding site	97	1	Substrate By similarity
	Site	182	1	Important for dimerization By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1VN15 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 686D38EF6C3B5C06	FASTA	307	35,246
	10 20 30 40 50 60 MSSIDLSTVR TFLLGLQERI THAIAEVDGQ PFMTDHWQKE PGETLQGNGI TKILEQGRVF 70 80 90 100 110 120 ERAGCGFSHV RGPRLPPSAT QHRPELAGAA FEAMGVSLVF HPRNPYVPTV HMNVRMLAAT 130 140 150 160 170 180 PVGENCEPVC WFGGGMDLTP FYGFDEDAVH FHQVCKDSLK PFGDDKYPRF KQWCDEYFYL 190 200 210 220 230 240 KHRQEQRGIG GVFFDDFQEL GLAQSFAMMQ SVGDSFLQAY LPIVERRKDS LFGERERDFQ 250 260 270 280 290 300 LYRRGRYVEF NLVWDRGTHF GLQSGGRTES ILMSMPPLAS WSYQRPNEAG SPEERLYKEF LVRRDWV

References

[1]

"Complete sequence of chromosome 1 of Polaromonas naphthalenivorans CJ2." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D.R., Brettin T., Bruce D., Han C., Tapia R., Brainard J., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Madsen E.L., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CJ2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000529 Genomic DNA. Translation: ABM37043.1.
RefSeq	YP_981964.1. NC_008781.1.
3D structure databases
ProteinModelPortal	A1VN15.
SMR	A1VN15. Positions 2-307.
ModBase	Search...
Protein-protein interaction databases
STRING	A1VN15.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4687031.
GenomeReviews	Gene locus Pnap_1730 in contig CP000529_GR.
KEGG	pna:Pnap_1730.
PATRIC	22948088. VBIPolNap76733_2591.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0408.
HOGENOM	HBG631180.
OMA	VKAYLLD.
PhylomeDB	A1VN15.
ProtClustDB	PRK05330.
Enzyme and pathway databases
BioCyc	PNAP365044:PNAP_1730-MONOMER.
Family and domain databases
HAMAP	MF_00333. Coprogen_oxidas. [Tree]
InterPro	IPR001260. Coprogen_oxidase_aer. IPR018375. Coprogen_oxidase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1500.10. Coprogen_oxidas. 1 hit.
KO	K00228.
PANTHER	PTHR10755. Coprogen_oxidas. 1 hit.
Pfam	PF01218. Coprogen_oxidas. 1 hit. [Graphical view]
PIRSF	PIRSF000166. Coproporphyri_ox. 1 hit.
PRINTS	PR00073. COPRGNOXDASE.
SUPFAM	SSF102886. Coprogen_oxidas. 1 hit.
PROSITE	PS01021. COPROGEN_OXIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM6_POLNA

Accession

Primary (citable) accession number: A1VN15

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program