A1VMA8 (GUAC_POLNA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP reductase
EC=1.7.1.7

Alternative name(s):
Guanosine 5'-monophosphate oxidoreductase
Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	Pnap_1472

Organism

Polaromonas naphthalenivorans (strain CJ2) [Complete proteome] [HAMAP]

Taxonomic identifier

365044 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Polaromonas

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity. HAMAP MF_01511
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_01511
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	GMP reductase HAMAP MF_01511		PRO_0000294281
Regions
	Nucleotide binding	202 – 225	24	NADP Potential
Sites
	Active site	173	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1VMA8 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: F79439DE86C2F2DF	FASTA	325	35,940
	10 20 30 40 50 60 MEIFDYDNIL LLPRKCRVES RAECDASVTL GQRSFRIPVV PANMKTVVNE DICVWMAQNG 70 80 90 100 110 120 YFYVMHRFDL DNLQFVRDMQ ARGVYASISL GVKQPDYDTV DRLVAEGLAP EYITIDIAHG 130 140 150 160 170 180 HADTVRKMIA YLKNHLPESF VIAGNVATPE AVIDLENWGA DATKVGIGPG KVCITKMKTG 190 200 210 220 230 240 FGTGGWQLSA LKWCARVATK PIIADGGIRE HGDIAKSIRF GATMVMIGSM LAGLEESPGQ 250 260 270 280 290 300 TVEVDGKLFK EYYGSASDFN KGEYKHVEGK RILEPIKGKL VDTLREMEED VQSAISYAGG 310 320 TRLMDIRKVN YVVLGGKNSN EDLMM

References

[1]

"Complete sequence of chromosome 1 of Polaromonas naphthalenivorans CJ2." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D.R., Brettin T., Bruce D., Han C., Tapia R., Brainard J., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Madsen E.L., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CJ2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000529 Genomic DNA. Translation: ABM36786.1.
RefSeq	YP_981707.1. NC_008781.1.
3D structure databases
ProteinModelPortal	A1VMA8.
SMR	A1VMA8. Positions 3-319.
ModBase	Search...
Protein-protein interaction databases
STRING	A1VMA8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4689530.
GenomeReviews	Gene locus Pnap_1472 in contig CP000529_GR.
KEGG	pna:Pnap_1472.
PATRIC	22947551. VBIPolNap76733_2325.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0516.
HOGENOM	HBG298985.
OMA	PDYITID.
PhylomeDB	A1VMA8.
ProtClustDB	PRK05458.
Enzyme and pathway databases
BioCyc	PNAP365044:PNAP_1472-MONOMER.
Family and domain databases
HAMAP	MF_01511. GMP_reduct_type2. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K00364.
PANTHER	PTHR11911:SF30. PTHR11911:SF30. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_POLNA

Accession

Primary (citable) accession number: A1VMA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program