true2008-05-202024-03-27101HEM1_POLNAThe genome of Polaromonas naphthalenivorans strain CJ2, isolated from coal tar-contaminated sediment, reveals physiological and metabolic versatility and evolution through extensive horizontal gene transfer.Yagi J.M.Sims D.Brettin T.Bruce D.Madsen E.L.doi:10.1111/j.1462-2920.2009.01947.x2009Environ. Microbiol.112253-2270NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]CJ2BacteriaNAD_bind_Glutamyl_tRNA_reductGlutamyl-tRNA reductase, N-terminal domainNAD(P)-binding Rossmann-like DomainGlu_tRNA_reductase4pyrrol_synth_GluRdtase4pyrrol_synth_GluRdtase_dimer4pyrrol_synth_GluRdtase_NGluRdtase_CSGluRdtase_dimer_dom_sfGluRdtase_N_sfNAD(P)-bd_dom_sfShikm_DH/Glu-tRNA_RdtasehemAGLUTAMYL-TRNA REDUCTASEGLUTAMYL-TRNA REDUCTASEGlutR_dimerGlutR_NShikimate_DH4pyrrol_synth_GluRdtaseGlutamyl tRNA-reductase catalytic, N-terminal domainGlutamyl tRNA-reductase dimerization domainNAD(P)-binding Rossmann-fold domainsGLUTRGlutamyl-tRNA reductaseGluTR1.2.1.70hemAPnap_0776Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).Homodimer.Possesses an unusual extended V-shaped dimeric structure with each monomer consisting of three distinct domains arranged along a curved 'spinal' alpha-helix. The N-terminal catalytic domain specifically recognizes the glutamate moiety of the substrate. The second domain is the NADPH-binding domain, and the third C-terminal domain is responsible for dimerization.During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA.Belongs to the glutamyl-tRNA reductase family.Glutamyl-tRNA reductase467541429Nucleophile515053108113115119188193Important for activity98substrate2007-02-06146754a1c43c341454d2bab6c712d3e7abba0dMSVWALGLNHHTAPLDLRGRFAFAIDQIEPTLRGLRASLARQPEATLLSTCNRTEIYCAGDTNDLDSTMEWLAHNGGVSPSLLRAHAYTLQDDQAARHAFRVASGLDSMVLGEPQILGQMKDAVRAAEDAGAMGTTLHQLFQRSFAVAKEVRTSTEIGAHSISMAAAAVRLAGQLFEDLGDIKVLFVGAGEMIDLAATHFAAKNPKAMAIANRSLDRGEKLASRFGAEVMRLGDLPGRLHEFDAVISCTASTLPIIGLGAVERALKRRKHRPMFMVDLAVPRDIEPEVKELSDIYLYTVDDLAHVVQTGRDSRQAAVAEAEVIIDAGVQNFMHWLDQRGSVPLIQQLNAQADAWRAAEIVRAKKLLARGESIEEVLEAMSRGLTQKMLHGAMAELHAGDASSREATARTVSKLFLRGQMPRAATERKERtrue