Reviewed, UniProtKB/Swiss-Prot A1VJF1 (ACEK_POLNA)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	Pnap_0457

Organism

Polaromonas naphthalenivorans (strain CJ2) [Complete proteome] [HAMAP]

Taxonomic identifier

365044 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Polaromonas

Protein attributes

Sequence length	623 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity.
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 623	623	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000288291
Regions
	Nucleotide binding	344 – 350	7	ATP By similarity
Sites
	Active site	400	1	By similarity
	Binding site	365	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1VJF1-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: C690BC675EB52FDE	FASTA	623	72,058
	10 20 30 40 50 60 MFPQRLDSTV AYAIAKAMID GFNRHYRLFR TESARAKHRF ETADWHGQQR AQRERIEFYD 70 80 90 100 110 120 LRVREASIRL EREFKAGEQP MDVWHQVKLH YIGLLVNHHQ PELAETFFNS VTTKILHRSY 130 140 150 160 170 180 FQNDFIFVRP AVSTEYIENQ EPTAQPTYRA YYPTQGNMHE TLVKLVEDFG LCREFEDLRR 190 200 210 220 230 240 DAGYVAEAMA ARLGDVKLRA NFQIQVLSGL FFRNKGAYVV GKIINGFGEI PFALPILHRQ 250 260 270 280 290 300 PAPGQVLPAD GKGISSQSDA GKLVIDAALF GEDDLLILFS FARAYFMVDM GIPSAFVQFL 310 320 330 340 350 360 RSMMPRMPRA EIYNALGLAK QGKTLFYRDF LHHLRHSTDK FRIAPGIKGM VMLVFDLPSF 370 380 390 400 410 420 PYVFKVIKDY YPPQKDTTRE QIKGKYLLVK QHDRVGRMAD TQEYSEVAFP RERFSDELIA 430 440 450 460 470 480 EIEKFAPSQL EISDRDGDGQ MEVIIKHVYI ERRMIPLNIY LQEAFDFGVA EPAARDQIER 490 500 510 520 530 540 AVVEYGNAIK DMVAANIFPG DMLWKNFGIT RHGKVVFYDY DEIEYITDCK FRKVPTPRNE 550 560 570 580 590 600 EDEMSGEVWY SVGPKDVFPE TFGPFLLGND AVREVFMKHH ADLLDAAFWQ RHQARIQAGH 610 620 VYDVFPYDQQ KRFAVMHGPA RAG

References

[1]

"Complete sequence of chromosome 1 of Polaromonas naphthalenivorans CJ2." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D.R., Brettin T., Bruce D., Han C., Tapia R., Brainard J., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Madsen E.L., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000529 Genomic DNA. Translation: ABM35779.1.
RefSeq	YP_980700.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1VJF1.
Genome annotation databases
GeneID	4688787.
GenomeReviews	Gene locus Pnap_0457 in contig CP000529_GR.
KEGG	pna:Pnap_0457.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FYRDFLH.
Family and domain databases
HAMAP	MF_00747. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACEK_POLNA

Accession

Primary (citable) accession number: A1VJF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)