A1VIH8 (ALR_POLNA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase
EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	Pnap_0131

Organism

Polaromonas naphthalenivorans (strain CJ2) [Complete proteome] [HAMAP]

Taxonomic identifier

365044 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Polaromonas

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	Alanine racemase HAMAP MF_01201		PRO_1000066023
Sites
	Active site	35	1	Proton acceptor; specific for D-alanine By similarity
	Active site	256	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	35	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1VIH8 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 81724396533FB6CA	FASTA	365	39,536
	10 20 30 40 50 60 MPRPILATIH LAALSHNLAR VRTAAPDAKV WAVVKANAYG HGIERVFDAL RSADGFALLD 70 80 90 100 110 120 LDEARRLRAL DWRGPILLLE GCFEARDLEL CSRLGLWHTI HCQEQIDMLA AHKTQLPHRV 130 140 150 160 170 180 FLKMNTGMNR LGFTPERFRA AWIRLNALPQ VDEISLMQHF PDADGPKGIT AQLQAFDAVT 190 200 210 220 230 240 HDLPGERTLS NSAAILRHTG ALAGRSDWVR PGIVLYGSAP DFPEHSASDW ELQPTMTLSS 250 260 270 280 290 300 RIIGLQTLAA GDTVGYGSSF VAEGPMRIGV VACGYADGYP RHCPTGTPVL VNGVRTRMVG 310 320 330 340 350 360 RVSMDMITVD LTPVPRAGMG SEVTLWGRAS KGGAMLPIDE VARAAGTVGY ELMCAVAPRV PVAVD

References

[1]

"Complete sequence of chromosome 1 of Polaromonas naphthalenivorans CJ2." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D.R., Brettin T., Bruce D., Han C., Tapia R., Brainard J., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Madsen E.L., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CJ2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000529 Genomic DNA. Translation: ABM35456.1.
RefSeq	YP_980377.1. NC_008781.1.
3D structure databases
ProteinModelPortal	A1VIH8.
ModBase	Search...
Protein-protein interaction databases
STRING	A1VIH8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4689202.
GenomeReviews	Gene locus Pnap_0131 in contig CP000529_GR.
KEGG	pna:Pnap_0131.
PATRIC	22944739. VBIPolNap76733_0940.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0787.
HOGENOM	HBG712172.
OMA	SVELWGD.
PhylomeDB	A1VIH8.
ProtClustDB	CLSK951002.
Enzyme and pathway databases
BioCyc	PNAP365044:PNAP_0131-MONOMER.
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Gene3D	G3DSA:2.40.37.10. Ala_racemase/Decarboxylase_C. 1 hit.
KO	K01775.
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. Alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_POLNA

Accession

Primary (citable) accession number: A1VIH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program