Reviewed, UniProtKB/Swiss-Prot A1VDX6 (ISPDF_DESVV)

Names and origin

Protein names

Recommended name:
    Bifunctional enzyme ispD/ispF
Including the following 2 domains:
    1- Recommended name:
            2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
              EC=2.7.7.60
        Alternative name(s):
            4-diphosphocytidyl-2C-methyl-D-erythritol synthase
            MEP cytidylyltransferase
              Short name=MCT
    2- Recommended name:
            2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
                Short name=MECPS
                Short name=MECDP-synthase
              EC=4.6.1.12

Gene names

Name:	ispDF
Ordered Locus Names:	Dvul_1625

Organism

Desulfovibrio vulgaris subsp. vulgaris (strain DP4) [Complete proteome] [HAMAP]

Taxonomic identifier

391774 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Desulfovibrio

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (ispD), and converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP (ispF) By similarity.
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_01520 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP. HAMAP MF_01520
Cofactor	Divalent metal cations By similarity.
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_01520 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.
Sequence similarities	In the N-terminal section; belongs to the ispD family. In the C-terminal section; belongs to the ispF family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding
Molecular function	Lyase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity Inferred from electronic annotation. Source: HAMAP 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	Bifunctional enzyme ispD/ispF HAMAP MF_01520		PRO_0000296744
Regions
	Region	1 – 237	237	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_01520
	Region	238 – 395	158	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase HAMAP MF_01520
Sites
	Metal binding	244	1	Divalent metal cation By similarity
	Metal binding	246	1	Divalent metal cation By similarity
	Metal binding	278	1	Divalent metal cation By similarity
	Site	15	1	Transition state stabilizer By similarity
	Site	25	1	Transition state stabilizer By similarity
	Site	161	1	Positions MEP for the nucleophilic attack By similarity
	Site	217	1	Positions MEP for the nucleophilic attack By similarity
	Site	270	1	Transition state stabilizer By similarity
	Site	369	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1VDX6-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: B21D4C2D48587876	FASTA	395	41,726
	10 20 30 40 50 60 MRTWVLLLAA GSGTRLATAG LPDAKQFLPL HGAPLYWASA RTMAHVAGIE GIVFVFPPHR 70 80 90 100 110 120 VEEERARISA LDDGSVLGLD WHVVGGGAAR QDSVACGLAA LPRSCEAVLV HDAARPFASP 130 140 150 160 170 180 ALVARVLSAL HDGHAGVVPG IPLTDTVKET TDGFVANTPD RSRLVAVQTP QGFTLKALST 190 200 210 220 230 240 AHETARTAGW NVTDDAALLE RCGIPVRIVA GEVVNAKITT PEDLAMLDAN EPQVTVPCVG 250 260 270 280 290 300 WGYDVHRYGE GRPMKLGGVL IPEGPEVVAH SDGDVLLHAL ADALLGCIGA GDIGLHFPDS 310 320 330 340 350 360 DAAFDNANSA MLLDRVLHMT HEARLRLTHV DLTIVAQVPK LSPWRDKIRA NVARLLDLPV 370 380 390 TSVNFKATTE EGLGFTGEKR GIKAIAAVTG LRPMP

References

[1]

"Complete sequence of chromosome 1 of Desulfovibrio vulgaris subsp. vulgaris DP4." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Lykidis A., Walker C., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000527 Genomic DNA. Translation: ABM28642.1.
RefSeq	YP_967069.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1VDX6.
Genome annotation databases
GeneID	4664257.
GenomeReviews	Gene locus Dvul_1625 in contig CP000527_GR.
KEGG	dvl:Dvul_1625.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	IVLIHDA.
Family and domain databases
HAMAP	MF_01520. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. IPR003526. MECDP_synthase_core. IPR020555. MECDP_synthase_CS. [Graphical view]
Gene3D	G3DSA:3.30.1330.50. MECDP_synthase_core. 1 hit.
Pfam	PF01128. IspD. 1 hit. PF02542. YgbB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. ispD. 1 hit. TIGR00151. ispF. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. PS01350. ISPF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPDF_DESVV

Accession

Primary (citable) accession number: A1VDX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)