SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A1VCU3

- SYI_DESVV

UniProt

A1VCU3 - SYI_DESVV

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Isoleucine--tRNA ligase

Gene
ileS, Dvul_1240
Organism
Desulfovibrio vulgaris subsp. vulgaris (strain DP4)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei566 – 5661Aminoacyl-adenylate By similarity
Binding sitei610 – 6101ATP By similarity
Metal bindingi906 – 9061Zinc By similarity
Metal bindingi909 – 9091Zinc By similarity
Metal bindingi926 – 9261Zinc By similarity
Metal bindingi929 – 9291Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciDVUL391774:GHS0-1283-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Dvul_1240
OrganismiDesulfovibrio vulgaris subsp. vulgaris (strain DP4)
Taxonomic identifieri391774 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeDesulfovibrio
ProteomesiUP000009173: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 938938Isoleucine--tRNA ligaseUniRule annotationPRO_1000070886Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi391774.Dvul_1240.

Structurei

3D structure databases

ProteinModelPortaliA1VCU3.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotationAdd
BLAST
Motifi607 – 6115"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1VCU3-1 [UniParc]FASTAAdd to Basket

« Hide

MSDYKKTLQL PETKFPMKAN LTQREPEMLR KWEKDDAYGA MVRASGQQGT    50
YVLHDGPPYA NGNIHMGHAL NKILKDIIVK SRNLQGFKAE YVPGWDCHGL 100
PIELKVEHEL GEKKRTMPAH AVRKRCRQYA EKYLDIQRKE FKRLGVFGAW 150
DKPYVTMHPS YEAATARELG NFAAKGGLVR SKKPIYWCCS CQTALAEAEV 200
EYHDHTSPSV HVRFPLRDPR VAEVLPGVDP AHAYIVIWTT TPWTLPDNMA 250
VAVHPDFDYV VVRHGGDFHI VAEGLLEACL KAFKWDEHEV VARIGGRALE 300
GLKATHPFYD RPSPIVLADY VTLESGTGCV HTAPGHGRED YETGLRYGLD 350
IYSPLTDEGR YLDCVEFFAG MTIFEANPKV IEKLREVGNL LAEGRITHSY 400
PHCWRCKKPV IFRATTQWFI AMERNDLRQK ALDAIRDDVR WIPSWGQERI 450
HNMIEFRPDW CISRQRMWGV PIVALLCEDC GEAWNDADWM RDIAERFAKH 500
ATGCDYWYET DLSDIVPAGL RCPKCGGDHW KKETDILDVW FDSGTSFAAV 550
VEQREECGFP ADLYLEGSDQ HRGWFHSSLL ASIGTRGVPP YRSVLTHGYV 600
VDGDGRKMSK SVGNVVAPQE IIDKHGAEVL RLWVASVDYR EDIRISEEIL 650
NRLVDAYRRI RNTCRYLLGN ISDLTPETMV PFEAMDPLDR FALDLASRAH 700
ERIQDAYTEY EFHKVFHTLH NLCVTDLSAF YLDILKDRLY SSAADSHARR 750
SAQTALYRIL MLMVRDMAPV LSFTAEEVFG YVPAALRPDV ISVFALPATD 800
APAFTLDTTS RAAWEKLLAV RSETTKAIEP LRKSGEVGHS LDTHVTLFAD 850
PSLKATLEGL GSDLRAMFIV SRLEVMNLAD APADAWTSEE LPELRVAVRK 900
AEGEKCERCW IISADLGTDA AHPTLCPRCT AVLTGTGA 938
Length:938
Mass (Da):105,975
Last modified:February 6, 2007 - v1
Checksum:i4B531856C7308EFE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000527 Genomic DNA. Translation: ABM28259.1.
RefSeqiWP_011792149.1. NC_008751.1.
YP_966686.1. NC_008751.1.

Genome annotation databases

EnsemblBacteriaiABM28259; ABM28259; Dvul_1240.
GeneIDi4663467.
KEGGidvl:Dvul_1240.
PATRICi21766305. VBIDesVul62463_1438.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000527 Genomic DNA. Translation: ABM28259.1 .
RefSeqi WP_011792149.1. NC_008751.1.
YP_966686.1. NC_008751.1.

3D structure databases

ProteinModelPortali A1VCU3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 391774.Dvul_1240.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABM28259 ; ABM28259 ; Dvul_1240 .
GeneIDi 4663467.
KEGGi dvl:Dvul_1240.
PATRICi 21766305. VBIDesVul62463_1438.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci DVUL391774:GHS0-1283-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DP4.

Entry informationi

Entry nameiSYI_DESVV
AccessioniPrimary (citable) accession number: A1VCU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 6, 2007
Last modified: September 3, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi