Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A1VB97

- BIOB_DESVV

UniProt

A1VB97 - BIOB_DESVV

Protein

Biotin synthase

Gene

bioB

Organism
Desulfovibrio vulgaris subsp. vulgaris (strain DP4)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (06 Feb 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi104 – 1041Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi108 – 1081Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi111 – 1111Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi180 – 1801Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi240 – 2401Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciDVUL391774:GHS0-715-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:Dvul_0690
    OrganismiDesulfovibrio vulgaris subsp. vulgaris (strain DP4)
    Taxonomic identifieri391774 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeDesulfovibrio
    ProteomesiUP000009173: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 361361Biotin synthasePRO_0000381353Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi391774.Dvul_0690.

    Structurei

    3D structure databases

    ProteinModelPortaliA1VB97.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239958.
    KOiK01012.
    OMAiDETQALC.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A1VB97-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHNLLENLRR RLLAQRTPEP LPPTSQGLAR PSHDVVRGPC DADIPPDARN    50
    TMPEGITVEE ALAVAELPQK HALDILATAQ AIRSVHKGGP AALCGIVNAK 100
    SGRCPEDCAF CAQSSHHATG SPVHALLDAE TLLRRAEELR QSGAERYGIV 150
    TSGTRLTVRE LDTLCEAAVR IRRETGIALC GSLGQLTPDA AACLKEAGFS 200
    SYHHNLETSR SFFPAICSTH AYDDDIATVR AARAAGLRTC SGGIFGMGET 250
    DAQRIELSAT LRELDVDSIP VNLLSPIPGT PLQHRPTMPP MRALVSIAIY 300
    RLMHPARDIL VCGGREATLG PWQSWIFLAG ANGMMVGNYL TTTGRDMADD 350
    LAMLATLGVR A 361
    Length:361
    Mass (Da):38,644
    Last modified:February 6, 2007 - v1
    Checksum:iA0B7244C5658EC33
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000527 Genomic DNA. Translation: ABM27713.1.
    RefSeqiWP_011791775.1. NC_008751.1.
    YP_966140.1. NC_008751.1.

    Genome annotation databases

    EnsemblBacteriaiABM27713; ABM27713; Dvul_0690.
    GeneIDi4663563.
    KEGGidvl:Dvul_0690.
    PATRICi21765139. VBIDesVul62463_0874.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000527 Genomic DNA. Translation: ABM27713.1 .
    RefSeqi WP_011791775.1. NC_008751.1.
    YP_966140.1. NC_008751.1.

    3D structure databases

    ProteinModelPortali A1VB97.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 391774.Dvul_0690.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABM27713 ; ABM27713 ; Dvul_0690 .
    GeneIDi 4663563.
    KEGGi dvl:Dvul_0690.
    PATRICi 21765139. VBIDesVul62463_0874.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239958.
    KOi K01012.
    OMAi DETQALC.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci DVUL391774:GHS0-715-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DP4.

    Entry informationi

    Entry nameiBIOB_DESVV
    AccessioniPrimary (citable) accession number: A1VB97
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: February 6, 2007
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3