Reviewed,
UniProtKB/Swiss-Prot A1V7Z3 (GLMU_BURMS)
Last modified
November 3, 2009.
Version 22.
History...
Clusters with 100%,
90%,
50% identity |
 Documents (2) |
 Third-party data |
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Names and origin
| Protein names | Recommended name: Bifunctional protein glmU Including the following 2 domains: 1- Recommended name: UDP-N-acetylglucosamine pyrophosphorylase EC=2.7.7.23 Alternative name(s): N-acetylglucosamine-1-phosphate uridyltransferase 2- Recommended name: Glucosamine-1-phosphate N-acetyltransferase EC=2.3.1.157 | ||||
| Gene names |
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| Organism | Burkholderia mallei (strain SAVP1) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 320388 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group |
Protein attributes
| Sequence length | 453 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc By similarity. |
| Catalytic activity | Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate. HAMAP MF_01631 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-D-glucosamine. HAMAP MF_01631 |
| Cofactor | Binds 1 magnesium ion per subunit By similarity. |
| Pathway | Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II): step 2/2. HAMAP MF_01631 Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_01631 |
| Subcellular location | Cytoplasm By similarity. |
| Sequence similarities | In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family. In the C-terminal section; belongs to the transferase hexapeptide repeat family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 453 | 453 | Bifunctional protein glmU HAMAP MF_01631 | PRO_1000056143 | |||||
Regions | |||||||||
| Region | 1 – 225 | 225 | Pyrophosphorylase By similarity | ||||||
| Region | 6 – 9 | 4 | Substrate binding By similarity | ||||||
| Region | 76 – 77 | 2 | Substrate binding By similarity | ||||||
| Region | 226 – 246 | 21 | Linker By similarity | ||||||
| Region | 247 – 453 | 207 | N-acetyltransferase By similarity | ||||||
Sites | |||||||||
| Active site | 359 | 1 | Proton acceptor By similarity | ||||||
| Metal binding | 100 | 1 | Magnesium By similarity | ||||||
| Metal binding | 223 | 1 | Magnesium By similarity | ||||||
| Binding site | 71 | 1 | Substrate By similarity | ||||||
| Binding site | 135 | 1 | Substrate; via amide nitrogen By similarity | ||||||
| Binding site | 150 | 1 | Substrate By similarity | ||||||
| Binding site | 165 | 1 | Substrate By similarity | ||||||
| Binding site | 383 | 1 | Acetyl-CoA By similarity | ||||||
| Binding site | 401 | 1 | Acetyl-CoA By similarity | ||||||
| Binding site | 419 | 1 | Acetyl-CoA; via amide nitrogen By similarity | ||||||
Sequences
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References
| [1] | DeShazer D., Woods D.E., Nierman W.C. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
Cross-references
Sequence databases | |
|---|---|
| CP000526 Genomic DNA. Translation: ABM52634.1. | |
| RefSeq | YP_994346.1. |
3D structure databases | |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 4681393. |
| GenomeReviews | Gene locus BMASAVP1_A3051 in contig CP000526_GR. |
| KEGG | bmv:BMASAVP1_A3051. |
| TIGR | BMASAVP1_A3051. |
Phylogenomic databases | |
| OMA | TRMKSKL. |
Family and domain databases | |
| HAMAP | MF_01631. [Tree] |
| InterPro | IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR001228. ISPD_synthase. IPR005882. UDP_GlcNAc_PyrPase. [Graphical view] |
| Pfam | PF00132. Hexapep. 6 hits. PF01128. IspD. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01173. glmU. 1 hit. |
| PROSITE | PS00101. HEXAPEP_TRANSFERASES. False negative. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | GLMU_BURMS | ||||||||
| Accession | Primary (citable) accession number: A1V7Z3 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
