A1V193 (T23O_BURMS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan 2,3-dioxygenase
Short name=TDO
EC=1.13.11.11

Alternative name(s):
Tryptamin 2,3-dioxygenase
Tryptophan oxygenase
Short name=TO
Short name=TRPO
Tryptophan pyrrolase
Tryptophanase

Gene names

Name:	kynA
Ordered Locus Names:	BMASAVP1_A0650

Organism

Burkholderia mallei (strain SAVP1) [Complete proteome] [HAMAP]

Taxonomic identifier

320388 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Tryptophan 2,3-dioxygenase		PRO_0000360106
Regions
	Region	50 – 54	5	Substrate binding By similarity
	Region	75 – 79	5	Substrate binding By similarity
Sites
	Metal binding	264	1	Iron (heme axial ligand) By similarity
	Binding site	137	1	Substrate By similarity
	Binding site	141	1	Substrate By similarity
	Binding site	148	1	Heme By similarity
	Binding site	278	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1V193 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 2D8AB0B21020B95A	FASTA	306	34,902
	10 20 30 40 50 60 MQPPGDDAAP RCPFAGAHAP DAPHVPEAAG DDVQAGWHRA QLDFSQSMSY GDYLSLDPIL 70 80 90 100 110 120 DAQHPRSPDH NEMLFIIQHQ TSELWMKLAL YELRAALASI RDDALPPAFK MLARVSRVLE 130 140 150 160 170 180 QLVQAWNVLA TMTPSEYSAM RPYLGASSGF QSYQYRELEF ILGNKNAQML RPHAHRPAIH 190 200 210 220 230 240 AHLEASLQAP SLYDEVIRLL ARRGFPIAPE RLDADWTQPT RHDRTVETAW LAVYREPNAH 250 260 270 280 290 300 WELYEMAEEL VDLEDAFRQW RFRHVTTVER IIGFKQGTGS TSGAPYLRKM LDVVLFPELW HVRTTL

References

[1]	DeShazer D., Woods D.E., Nierman W.C. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SAVP1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000526 Genomic DNA. Translation: ABM51851.1.
RefSeq	YP_991996.1. NC_008785.1.
3D structure databases
ProteinModelPortal	A1V193.
SMR	A1V193. Positions 37-306.
ModBase	Search...
Protein-protein interaction databases
STRING	A1V193.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4679695.
GenomeReviews	Gene locus BMASAVP1_A0650 in contig CP000526_GR.
KEGG	bmv:BMASAVP1_A0650.
PATRIC	19154048. VBIBurMal134057_2291.
TIGR	BMASAVP1_A0650.
Phylogenomic databases
HOGENOM	HBG647485.
OMA	QYREIEF.
ProtClustDB	CLSK2391472.
Enzyme and pathway databases
BioCyc	BMAL320388:BMASAVP1_A0650-MONOMER.
Family and domain databases
InterPro	IPR017485. Trp_2-3-dOase_bac. IPR004981. Trp_2_3_dOase. [Graphical view]
KO	K00453.
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
TIGRFAMs	TIGR03036. Trp_2_3_diox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T23O_BURMS

Accession

Primary (citable) accession number: A1V193

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program