A1V0P0 (PROB_BURMS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate 5-kinase
EC=2.7.2.11

Alternative name(s):
Gamma-glutamyl kinase
Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	BMASAVP1_A0443

Organism

Burkholderia mallei (strain SAVP1) [Complete proteome] [HAMAP]

Taxonomic identifier

320388 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity HAMAP MF_00456.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Glutamate 5-kinase HAMAP MF_00456		PRO_1000081045
Regions
	Domain	280 – 358	79	PUA

Sequences

	Sequence		Length	Mass (Da)
	A1V0P0 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 614D38AC8CBA8C19	FASTA	372	39,233
	10 20 30 40 50 60 MRSIIADSKR LVVKVGSSLV TNDGRGLDHD AIGRWAAQIA ALRGAGKEVV LVSSGAIAEG 70 80 90 100 110 120 MQRLGWSKRP REIDELQAAA AVGQMGLAQV YESRFAEHGI RTAQILLTHA DLADRERYLN 130 140 150 160 170 180 ARSTLLTLLR LGVVPIINEN DTVVTDEIKF GDNDTLGALV ANLIEGDTLV ILTDQPGLFT 190 200 210 220 230 240 ADPRKDPGAT LVAEASAGAP ELEAMAGGAG SSIGRGGMLT KILAAKRAAH SGANTVIASG 250 260 270 280 290 300 RERDVLVRLA AGEAIGTQLI ARTARMAARK QWMADHLQVR GHVVIDAGAV DKLTAGGKSL 310 320 330 340 350 360 LPIGVVAVQG VFARGEVIAC VDDTGREVAR GITNYSSAET KLIQRKPSGE IETVLGYMLE 370 PELIHRDNLV LV

References

[1]	DeShazer D., Woods D.E., Nierman W.C. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SAVP1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000526 Genomic DNA. Translation: ABM51617.1.
RefSeq	YP_991793.1. NC_008785.1.
3D structure databases
ProteinModelPortal	A1V0P0.
ModBase	Search...
Protein-protein interaction databases
STRING	A1V0P0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4681757.
GenomeReviews	Gene locus BMASAVP1_A0443 in contig CP000526_GR.
KEGG	bmv:BMASAVP1_A0443.
PATRIC	19153628. VBIBurMal134057_2083.
TIGR	BMASAVP1_A0443.
Phylogenomic databases
HOGENOM	HBG507643.
OMA	TFGDNDM.
ProtClustDB	PRK05429.
Enzyme and pathway databases
BioCyc	BMAL320388:BMASAVP1_A0443-MONOMER.
Family and domain databases
HAMAP	MF_00456. ProB. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. IPR011529. Glu_5kinase. IPR005715. Glu_5kinase/COase_Synthase. IPR019797. Glutamate_5-kinase_CS. IPR002478. PUA. IPR015947. PUA-like_domain. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00931.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF88697. PUA-like. 1 hit.
TIGRFAMs	TIGR01027. ProB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_BURMS

Accession

Primary (citable) accession number: A1V0P0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program