A1UZN5 (ISPE_BURMS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	BMASAVP1_A0086

Organism

Burkholderia mallei (strain SAVP1) [Complete proteome] [HAMAP]

Taxonomic identifier

320388 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 293	293	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000007822
Regions
	Nucleotide binding	99 – 109	11	ATP Potential
Sites
	Active site	16	1	By similarity
	Active site	141	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1UZN5 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: D4468CCD7D15D19B	FASTA	293	31,635
	10 20 30 40 50 60 MTDTTRSLRD CLAPAKLNLF LHITGRRPDG YHALQSVFQL LDWGDRLHFT LRDDGKVSRV 70 80 90 100 110 120 TDVPGVPEES DLVVRAASLL KAHAGATLGV DIEIDKRLPM GAGLGGGSSD AATTLLALNR 130 140 150 160 170 180 LWRLDLPRTT LQSLAVKLGA DVPFFVFGKN AFAEGIGEAL QAVELPARWF LVVTPRVHVP 190 200 210 220 230 240 TAAIFSEKSL TRDSKPITIT DFLAQRGIDA GWPDSFGRND MQPVVTSKYA EVAKVVEWFY 250 260 270 280 290 NLTPARMTGS GASVFAAFKS KADAEAAQAK LPAGWNSAVA ESMSEHPLFA FAS

References

[1]	DeShazer D., Woods D.E., Nierman W.C. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SAVP1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000526 Genomic DNA. Translation: ABM50827.1.
RefSeq	YP_991438.1. NC_008785.1.
3D structure databases
ProteinModelPortal	A1UZN5.
ModBase	Search...
Protein-protein interaction databases
STRING	A1UZN5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4678907.
GenomeReviews	Gene locus BMASAVP1_A0086 in contig CP000526_GR.
KEGG	bmv:BMASAVP1_A0086.
PATRIC	19152930. VBIBurMal134057_1735.
TIGR	BMASAVP1_A0086.
Phylogenomic databases
HOGENOM	HBG734593.
OMA	LNLFLHV.
ProtClustDB	PRK00343.
Enzyme and pathway databases
BioCyc	BMAL320388:BMASAVP1_A0086-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_BURMS

Accession

Primary (citable) accession number: A1UZN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program