Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

3-isopropylmalate dehydratase small subunit

Gene

leuD

Organism
Burkholderia mallei (strain SAVP1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

Pathwayi: L-leucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-isopropylmalate dehydratase small subunit (leuD), 3-isopropylmalate dehydratase large subunit (leuC)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00048; UER00071.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase small subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
Alternative name(s):
Alpha-IPM isomeraseUniRule annotation
Short name:
IPMIUniRule annotation
Isopropylmalate isomeraseUniRule annotation
Gene namesi
Name:leuDUniRule annotation
Ordered Locus Names:BMASAVP1_1642
OrganismiBurkholderia mallei (strain SAVP1)
Taxonomic identifieri320388 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000637431 – 2163-isopropylmalate dehydratase small subunitAdd BLAST216

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA1UZ33.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LeuD family. LeuD type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000222939.
KOiK01704.
OMAiAFTTHTG.

Family and domain databases

CDDicd01577. IPMI_Swivel. 1 hit.
Gene3Di3.20.19.10. 1 hit.
HAMAPiMF_01031. LeuD_type1. 1 hit.
InterProiIPR004431. 3-IsopropMal_deHydase_ssu.
IPR015937. Acoase/IPM_deHydtase.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
IPR033940. IPMI_Swivel.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00694. Aconitase_C. 1 hit.
[Graphical view]
SUPFAMiSSF52016. SSF52016. 1 hit.
TIGRFAMsiTIGR00171. leuD. 1 hit.

Sequencei

Sequence statusi: Complete.

A1UZ33-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKFNVHTGV VAPLDRENVD TDAIIPKQFL KSIKRTGFGP NAFDEWRYLD
60 70 80 90 100
HGEPGQDNSK RPLNPDFVLN QPRYQGASVL LARKNFGCGS SREHAPWALQ
110 120 130 140 150
QYGFRAIVAP SFADIFFNNC YKNGLLPIVL TEQQVDHLFN DTYAFNGYQL
160 170 180 190 200
TIDLDAQVVR APDGREYPFE ITAFRKYCLL NGFDDIGLTL RHADKIRQFE
210
AERLAKQPWL DNRLVG
Length:216
Mass (Da):24,726
Last modified:February 6, 2007 - v1
Checksum:i558A369A0E23F84F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000525 Genomic DNA. Translation: ABM49298.1.
RefSeqiWP_004187882.1. NC_008784.1.

Genome annotation databases

EnsemblBacteriaiABM49298; ABM49298; BMASAVP1_1642.
KEGGibmv:BMASAVP1_1642.
PATRICi19152526. VBIBurMal134057_1540.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000525 Genomic DNA. Translation: ABM49298.1.
RefSeqiWP_004187882.1. NC_008784.1.

3D structure databases

ProteinModelPortaliA1UZ33.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABM49298; ABM49298; BMASAVP1_1642.
KEGGibmv:BMASAVP1_1642.
PATRICi19152526. VBIBurMal134057_1540.

Phylogenomic databases

HOGENOMiHOG000222939.
KOiK01704.
OMAiAFTTHTG.

Enzyme and pathway databases

UniPathwayiUPA00048; UER00071.

Family and domain databases

CDDicd01577. IPMI_Swivel. 1 hit.
Gene3Di3.20.19.10. 1 hit.
HAMAPiMF_01031. LeuD_type1. 1 hit.
InterProiIPR004431. 3-IsopropMal_deHydase_ssu.
IPR015937. Acoase/IPM_deHydtase.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
IPR033940. IPMI_Swivel.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00694. Aconitase_C. 1 hit.
[Graphical view]
SUPFAMiSSF52016. SSF52016. 1 hit.
TIGRFAMsiTIGR00171. leuD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLEUD_BURMS
AccessioniPrimary (citable) accession number: A1UZ33
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 6, 2007
Last modified: November 30, 2016
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.