A1UU03 (LPXK_BARBK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetraacyldisaccharide 4'-kinase
EC=2.7.1.130

Alternative name(s):
Lipid A 4'-kinase

Gene names

Name:	lpxK
Ordered Locus Names:	BARBAKC583_1203

Organism

Bartonella bacilliformis (strain ATCC 35685 / KC583) [Complete proteome] [HAMAP]

Taxonomic identifier

360095 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA) By similarity. HAMAP MF_00409
Catalytic activity	ATP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate). HAMAP MF_00409
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6. HAMAP MF_00409
Sequence similarities	Belongs to the LpxK family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tetraacyldisaccharide 4'-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Tetraacyldisaccharide 4'-kinase HAMAP MF_00409		PRO_0000291195
Regions
	Nucleotide binding	53 – 60	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A1UU03 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 6BB46677A2CB2EFC	FASTA	339	37,660
	10 20 30 40 50 60 MYISAPHFWW KNKSFLSFLL TPFSKVYGYC ARRGMERKPP VIDLPVLCVG NFTLGGAGKT 70 80 90 100 110 120 PVVIVLSQAA KKLGLKPGVV SRGYGRTVKG VHLVDVECDD AYHVGDEPLL LARHALVAIS 130 140 150 160 170 180 PDRYAAAKRL KEEGCDLILM DDGFQSRRLY MDYALLVVDA MRGLGNGAVF PAGPLRAPLE 190 200 210 220 230 240 TQLSLMDSVL CIGHLKACNH VDAFITGTGK SLHYAHLKPL ASDEVSGKSF LAFAGIGNPD 250 260 270 280 290 300 KFFESIEEIS GHVIETCSYP DHYFFTDADL KNLKQKAKIQ NLWLATTAKD YTRIQAKALQ 310 320 330 KDIENLIVFD VTLDFAQADF CYRLLEEVIA RFKKRKHSL

References

[1]

Hendrix L., Mohamoud Y., Radune D., Shvartsbeyn A., Daugherty S., Dodson R., Durkin A.S., Harkins D., Huot H., Kothari S.P., Madupu R., Li J., Nelson W.C., Shrivastava S., Giglio M.G., Haft D., Selengut J., Fraser-Ligget C., Seshadri R. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35685 / KC583.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000524 Genomic DNA. Translation: ABM44776.1.
RefSeq	YP_989456.1. NC_008783.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1UU03.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4683863.
GenomeReviews	Gene locus BARBAKC583_1203 in contig CP000524_GR.
KEGG	bbk:BARBAKC583_1203.
NMPDR	fig|360095.3.peg.955.
PATRIC	20538717. VBIBarBac6912_1172.
TIGR	BARBAKC583_1203.
Phylogenomic databases
eggNOG	COG1663.
HOGENOM	HBG663914.
OMA	VILMDDG.
ProtClustDB	PRK00652.
Enzyme and pathway databases
BioCyc	BBAC360095:BARBAKC583_1203-MONOMER.
Family and domain databases
HAMAP	MF_00409. LpxK. [Tree]
InterPro	IPR003758. Tetraacyldisaccharide_4-kinase. [Graphical view]
KO	K00912.
Pfam	PF02606. LpxK. 1 hit. [Graphical view]
TIGRFAMs	TIGR00682. LpxK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXK_BARBK

Accession

Primary (citable) accession number: A1UU03

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program