A1UTE8 (CARA_BARBK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carbamoyl-phosphate synthase small chain
EC=6.3.5.5

Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	BARBAKC583_0972

Organism

Bartonella bacilliformis (strain ATCC 35685 / KC583) [Complete proteome] [HAMAP]

Taxonomic identifier

360095 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the CarA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 399	399	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_1000138848
Regions
	Domain	208 – 396	189	Glutamine amidotransferase type-1
	Region	1 – 204	204	CPSase HAMAP MF_01209
Sites
	Active site	285	1	Nucleophile By similarity
	Active site	369	1	By similarity
	Active site	371	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1UTE8 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: A3021250FB0402AE	FASTA	399	43,998
	10 20 30 40 50 60 MTKTTLSSDP WSIKKPTALL VLADGTVIEG EGIGAIGIVE AEVCFNTAIT GYEEILTDPS 70 80 90 100 110 120 YTGQIINFTF PHIGNVGTNS EDIEDLTPLH HYGAVGAIFK AHSSPSNYRA NENLNQWLKK 130 140 150 160 170 180 HQIIALCGID TRALTALIRE KGAQNAVIAH DPNGNFDINA LKERAQKWSG LLNLDLAKEV 190 200 210 220 230 240 TSKQSIEWNE KPWIWNKGYT INNEYNFHIV AIDYGIKRNI LRLMAAQGAR ITVVPAHTSV 250 260 270 280 290 300 QEILAMKPDG IFLSNGPGDP NATAEYAVPM IKTFIECNIP LFGICLGHQL LALAVGAKTI 310 320 330 340 350 360 KMHQGHHGAN HPVKNLITEK VEITSMNHGF TVDSTSLPQY VEETHISLFD STNCGIQIIG 370 380 390 KPAFSVQYHP EASPGPQDSH YLFQRFHDLI VNYREQSNK

References

[1]

Hendrix L., Mohamoud Y., Radune D., Shvartsbeyn A., Daugherty S., Dodson R., Durkin A.S., Harkins D., Huot H., Kothari S.P., Madupu R., Li J., Nelson W.C., Shrivastava S., Giglio M.G., Haft D., Selengut J., Fraser-Ligget C., Seshadri R. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35685 / KC583.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000524 Genomic DNA. Translation: ABM45277.1.
RefSeq	YP_989251.1. NC_008783.1.
3D structure databases
ProteinModelPortal	A1UTE8.
ModBase	Search...
Protein-protein interaction databases
STRING	A1UTE8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4684054.
GenomeReviews	Gene locus BARBAKC583_0972 in contig CP000524_GR.
KEGG	bbk:BARBAKC583_0972.
NMPDR	fig|360095.3.peg.573.
PATRIC	20538237. VBIBarBac6912_0942.
TIGR	BARBAKC583_0972.
Phylogenomic databases
eggNOG	COG0505.
HOGENOM	HBG286341.
OMA	FTYPELG.
ProtClustDB	PRK12564.
Enzyme and pathway databases
BioCyc	BBAC360095:BARBAKC583_0972-MONOMER.
Family and domain databases
HAMAP	MF_01209. CPSase_S_chain. [Tree]
InterPro	IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR017926. GATASE_1. [Graphical view]
Gene3D	G3DSA:3.50.30.20. G3DSA:3.50.30.20. 1 hit.
KO	K01956.
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
SMART	SM01097. CPSase_sm_chain. 1 hit. [Graphical view]
SUPFAM	SSF52021. CP_synthsmall. 1 hit.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_BARBK

Accession

Primary (citable) accession number: A1UTE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program