Reviewed, UniProtKB/Swiss-Prot A1UTC3 (MURB_BARBK)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylenolpyruvoylglucosamine reductase
    EC=1.1.1.158
Alternative name(s):
    UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	BARBAKC583_0945

Organism

Bartonella bacilliformis (strain ATCC 35685 / KC583) [Complete proteome] [HAMAP]

Taxonomic identifier

360095 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_1000002863
Regions
	Domain	39 – 220	182	FAD-binding PCMH-type
Sites
	Active site	185	1	By similarity
	Active site	234	1	Proton donor By similarity
	Active site	304	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1UTC3-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 79D4192C6A743B68	FASTA	324	35,898
	10 20 30 40 50 60 MNFQPIDGKK LLAWLQPVLS DIKGKITPNV EMRKVTWFRT GGLAELFYQP ADEDDLALFF 70 80 90 100 110 120 KVLPEFVPVT IVGIGSNLLV RDGGIPGVVI RLSTKSFGQI KQVSSTRFLV GAATADKHLA 130 140 150 160 170 180 SAALEAEISG FHFYHGIPGG LGGALKMNAG ANGVETAERV VEVYALDRKG ERHILNLRDM 190 200 210 220 230 240 NYAYRHCNIP EGLVFTAALL EGDVGNKDDI RAAMHEVALH RETVQPIREK TGGSTFRNLE 250 260 270 280 290 300 DISAWKVIDE AGCRGLQIGG AQMSEMHCNF MINMGEATGY DLEKLGETVR ARVFNHSAHH 310 320 LEWEIQRIGQ FEQDRIVLPF DQFH

References

[1]

Hendrix L., Mohamoud Y., Radune D., Shvartsbeyn A., Daugherty S., Dodson R., Durkin A.S., Harkins D., Huot H., Kothari S.P., Madupu R., Li J., Nelson W.C., Shrivastava S., Giglio M.G., Haft D., Selengut J., Fraser-Ligget C., Seshadri R. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000524 Genomic DNA. Translation: ABM44449.1.
RefSeq	YP_989226.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1UTC3.
Genome annotation databases
GeneID	4684962.
GenomeReviews	Gene locus BARBAKC583_0945 in contig CP000524_GR.
KEGG	bbk:BARBAKC583_0945.
NMPDR	fig|360095.3.peg.549.
TIGR	BARBAKC583_0945.
Phylogenomic databases
OMA	QPIREKT.
Family and domain databases
HAMAP	MF_00037. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00179. murB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_BARBK

Accession

Primary (citable) accession number: A1UTC3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)