A1USJ2 (SYT_BARBK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	BARBAKC583_0641

Organism

Bartonella bacilliformis (strain ATCC 35685 / KC583) [Complete proteome] [HAMAP]

Taxonomic identifier

360095 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	658 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00184
Subunit structure	Homodimer By similarity. HAMAP MF_00184
Subcellular location	Cytoplasm HAMAP MF_00184.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 658	658	Threonine--tRNA ligase HAMAP MF_00184		PRO_1000020343
Regions
	Region	246 – 549	304	Catalytic HAMAP MF_00184
Sites
	Metal binding	343	1	Zinc; catalytic By similarity
	Metal binding	394	1	Zinc; catalytic By similarity
	Metal binding	526	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1USJ2 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: DE4236DD7882B76D	FASTA	658	75,863
	10 20 30 40 50 60 MSFSISLSFP DGSQRDFPAE ITGLELAESI SKSLAKKAIA YSLNGVIRDL LDPLEQSGQV 70 80 90 100 110 120 EIITRDDPRA LQLIRHSCAH VLAEAVQELF PETQVTIGPV IENGFYYDFA RQQPFTLEDL 130 140 150 160 170 180 NTIEKKMREI IQRNKFFKKE IWSREKAKKI FSDKGELYKV ELIDAIPEDQ DLKIYYQGDW 190 200 210 220 230 240 FDLCRGPHVP STGQIGNAFK LMKVAGAYWR GDANNPMLTR IYGTAFSNEN ALKAYLNMLE 250 260 270 280 290 300 EAEKRDHRRL GREMDLFHFQ EEGPGMIFWH PKGWKMFQNL VSYMRRRLDE HKYDEVNAPQ 310 320 330 340 350 360 VLDKSLWETS GHWGWYQENM FKTIPATNDW NDEHVYALKP MNCPGHVQIF KHGLKSYRDL 370 380 390 400 410 420 PIRLAEFGLL HRYEPSGSLH GLMRVRSFTQ DDAHVFCTDE QLADECLKIN DLILSTYADF 430 440 450 460 470 480 GFEEIILKLS TRPEKRVGSD ELWDHAENIM MSVLKTIEKE AKGRIKTSIL QGEGAFYGPK 490 500 510 520 530 540 FEYTLKDAIG REWQCGTTQV DFNLPERFEV FYINRDSEKC QPVMIHRAIF GSMERFLGIL 550 560 570 580 590 600 IENFAGHMPL WLAPQQIVVT TITSEANEYA EKITAKLKAS GLSAVSDLRS EKINYKIREH 610 620 630 640 650 SLQKVPVILV CGKRESETNS VNMRRLGSMN QISLPIDQAI KQLTNEAIPP DLRRFMNS

References

[1]

Hendrix L., Mohamoud Y., Radune D., Shvartsbeyn A., Daugherty S., Dodson R., Durkin A.S., Harkins D., Huot H., Kothari S.P., Madupu R., Li J., Nelson W.C., Shrivastava S., Giglio M.G., Haft D., Selengut J., Fraser-Ligget C., Seshadri R. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35685 / KC583.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000524 Genomic DNA. Translation: ABM44590.1.
RefSeq	YP_988945.1. NC_008783.1.
3D structure databases
ProteinModelPortal	A1USJ2.
SMR	A1USJ2. Positions 245-653.
ModBase	Search...
Protein-protein interaction databases
STRING	A1USJ2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4684002.
GenomeReviews	Gene locus BARBAKC583_0641 in contig CP000524_GR.
KEGG	bbk:BARBAKC583_0641.
NMPDR	fig|360095.3.peg.321.
PATRIC	20537569. VBIBarBac6912_0625.
TIGR	BARBAKC583_0641.
Phylogenomic databases
eggNOG	COG0441.
HOGENOM	HBG352811.
OMA	VGSDALW.
ProtClustDB	PRK00413.
Enzyme and pathway databases
BioCyc	BBAC360095:BARBAKC583_0641-MONOMER.
Family and domain databases
HAMAP	MF_00184. Thr_tRNA_synth. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR012675. Beta-grasp_ferredoxin-type. IPR004095. TGS. IPR012676. TGS-like. IPR002320. Thr-tRNA-synth_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
KO	K01868.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF81271. TGS-like. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. ThrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_BARBK

Accession

Primary (citable) accession number: A1USJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program