A1USI4 (RISB_BARBK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6,7-dimethyl-8-ribityllumazine synthase
Short name=DMRL synthase
Short name=Lumazine synthase
EC=2.5.1.9

Alternative name(s):
Riboflavin synthase beta chain

Gene names

Name:	ribH
Ordered Locus Names:	BARBAKC583_0633

Organism

Bartonella bacilliformis (strain ATCC 35685 / KC583) [Complete proteome] [HAMAP]

Taxonomic identifier

360095 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate yielding 6,7-dimethyl-8-lumazine By similarity. HAMAP MF_00178
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine. HAMAP MF_00178
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2. HAMAP MF_00178
Sequence similarities	Belongs to the DMRL synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	riboflavin synthase complex Inferred from electronic annotation. Source: InterPro
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 152	152	6,7-dimethyl-8-ribityllumazine synthase HAMAP MF_00178		PRO_1000040373

Sequences

	Sequence		Length	Mass (Da)
	A1USI4 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 804FD3443822B135	FASTA	152	16,697
	10 20 30 40 50 60 MTQKLHINPH LLIVEARFYN EISDELLAGA VSVLQKSGVS YDIITVPGAL EIPAAIAFAE 70 80 90 100 110 120 KDKTVFYDGY VALGCVIRGE TYHFEIVADN SCRALMDLTV HQQLAIGNGI LTVENEKQAW 130 140 150 ARAKQGEKNK GDFAAEAALC MIALKKRFGD NR

References

[1]

Hendrix L., Mohamoud Y., Radune D., Shvartsbeyn A., Daugherty S., Dodson R., Durkin A.S., Harkins D., Huot H., Kothari S.P., Madupu R., Li J., Nelson W.C., Shrivastava S., Giglio M.G., Haft D., Selengut J., Fraser-Ligget C., Seshadri R. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35685 / KC583.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000524 Genomic DNA. Translation: ABM44740.1.
RefSeq	YP_988937.1. NC_008783.1.
3D structure databases
ProteinModelPortal	A1USI4.
SMR	A1USI4. Positions 9-149.
ModBase	Search...
Protein-protein interaction databases
STRING	A1USI4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4684624.
GenomeReviews	Gene locus BARBAKC583_0633 in contig CP000524_GR.
KEGG	bbk:BARBAKC583_0633.
NMPDR	fig|360095.3.peg.313.
PATRIC	20537553. VBIBarBac6912_0617.
TIGR	BARBAKC583_0633.
Phylogenomic databases
eggNOG	COG0054.
HOGENOM	HBG311126.
OMA	SSRALMD.
ProtClustDB	PRK00061.
Enzyme and pathway databases
BioCyc	BBAC360095:BARBAKC583_0633-MONOMER.
Family and domain databases
HAMAP	MF_00178. Lumazine_synth. [Tree]
InterPro	IPR002180. DMRL_synthase. [Graphical view]
Gene3D	G3DSA:3.40.50.960. DMRL_synthase. 1 hit.
KO	K00794.
PANTHER	PTHR21058. DMRL_synthase. 1 hit.
Pfam	PF00885. DMRL_synthase. 1 hit. [Graphical view]
SUPFAM	SSF52121. DMRL_synthase. 1 hit.
TIGRFAMs	TIGR00114. Lumazine-synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RISB_BARBK

Accession

Primary (citable) accession number: A1USI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program