A1USC8 (RPOB_BARBK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta
Short name=RNAP subunit beta
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta

Gene names

Name:	rpoB
Ordered Locus Names:	BARBAKC583_0571

Organism

Bartonella bacilliformis (strain ATCC 35685 / KC583) [Complete proteome] [HAMAP]

Taxonomic identifier

360095 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	1383 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01321
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01321
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1383	1383	DNA-directed RNA polymerase subunit beta HAMAP MF_01321		PRO_0000300282

Sequences

	Sequence		Length	Mass (Da)
	A1USC8 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 4B3588930FBD4021	FASTA	1,383	154,847
	10 20 30 40 50 60 MAQTLAMMSQ FNGRKRVRKF FGKIPEVAEM PNLIEVQKAS YDQFLMIKEP KGGRPDEGLQ 70 80 90 100 110 120 AVFKSVFPIS DFSGTAMLEF VRYEFDAPKF DVEECRQRDL TYAAPLKVIL RLIVFDIDED 130 140 150 160 170 180 TGSKDIKDIK EQGVYMGDMP LMTSNGTFII NGTERVIVSQ MHRSPGVFFD HDKGKSHSSG 190 200 210 220 230 240 KVLFAARVIP YRGSWLDIEF DAKDIVYARI DRRRKIPVTS FLMALGMDAS DILSTFYNKV 250 260 270 280 290 300 TYERVESGWR IPYSVDRFKG VKLVSDLVDA ESGEVVAEAG KKLTARTAKL LVEKGLKAIR 310 320 330 340 350 360 VSEDELLGSY LADDIVNYET GEIYLEAGDE IDEKVLKVLF DVGANQINIL DIDHMSVGAY 370 380 390 400 410 420 IRNTLKVDKN ESRQDALFDI YRVMRPGEPP TIDTAEAMFH SLFFDSERYD LSAVGRVKMN 430 440 450 460 470 480 LRMGLDCPDT VRVLRQEDII GVVKMLVELR DGRGEIDDID NLGNRRVRSV GELMENQYRV 490 500 510 520 530 540 GLLRMERAIK ERMSSVEIDT VMPQDLINAK PAAAAVREFF GSSQLSQFMD QTNPLSEITH 550 560 570 580 590 600 KRRLSALGPG GLTRERAGFE VRDVHPTHYG RICPIETPEG PNIGLINSLA TFARVNKYGF 610 620 630 640 650 660 IESPYRKITD GKVTTEVVYL SAMEEAKHYV AQANSSLDSE GRFTEEFVVC RHAGEVLMVP 670 680 690 700 710 720 RDHIDLMDVS PKQLVSVAAS LIPFLENDDA NRALMGSNMQ RQAVPLIRSE APFVGTGMEA 730 740 750 760 770 780 IVARDSGAAI SAKRGGIVDQ VDATRIVIRA TEDLDPSKSG VDIYRLQKFQ RSNQSTCINQ 790 800 810 820 830 840 RPLVHVGDRI EKGDIIADGP STDLGDLALG RNVLVAFMPW NGYNYEDSIL LSERIVADDV 850 860 870 880 890 900 FTSIHIEEFE VAARDMKLGP EEITRDIPNV AEEALRNLDE AGIVYIGAEV QPGDILVGKI 910 920 930 940 950 960 TPKGESPMTP EEKLLRAIFG EKASDVRDTS MRMPPGTFGT VVEVRVFNRH GVEKDERAME 970 980 990 1000 1010 1020 IEREEIERLA KDRDDEQSIL DRNVYARLAG MLKDKSAIRG PKGFEKGQKI DSTVLNNYPR 1030 1040 1050 1060 1070 1080 SQWWQFAVDD EKVQNKIEAL CNQYDESKEA LQRRFMDKVE KVQRGDEMPP GVMKMVKVFV 1090 1100 1110 1120 1130 1140 AVKRKIQPGD KMAGRHGNKG VVSRILPVED MPFLEDGTHA DIVLNPLGVP SRMNVGQILE 1150 1160 1170 1180 1190 1200 THLGWACAGM GKKIGDLVDF YQETGDILPL RQRIENLIPD NDRNEPVRQY DDESLVKLAH 1210 1220 1230 1240 1250 1260 QMRKGVSIAT PVFDGAHEAD INAMLEDAGL DSSGQVTLYD GRTGEPFDRQ VTVGYIYMLK 1270 1280 1290 1300 1310 1320 LHHLVDDKIH ARSIGPYSLV TQQPLGGKAQ FGGQRFGEME VWALEAYGAA YTLQEMLTVK 1330 1340 1350 1360 1370 1380 SDDVAGRTKV YEAIVRGDDT FEASIPESFN VLIKEMRSLG LNVKLGDARE FMAQQALPEV VEN

References

[1]

Hendrix L., Mohamoud Y., Radune D., Shvartsbeyn A., Daugherty S., Dodson R., Durkin A.S., Harkins D., Huot H., Kothari S.P., Madupu R., Li J., Nelson W.C., Shrivastava S., Giglio M.G., Haft D., Selengut J., Fraser-Ligget C., Seshadri R. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35685 / KC583.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000524 Genomic DNA. Translation: ABM45609.1.
RefSeq	YP_988881.1. NC_008783.1.
3D structure databases
ProteinModelPortal	A1USC8.
ModBase	Search...
Protein-protein interaction databases
STRING	A1USC8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4683812.
GenomeReviews	Gene locus BARBAKC583_0571 in contig CP000524_GR.
KEGG	bbk:BARBAKC583_0571.
NMPDR	fig|360095.3.peg.253.
PATRIC	20537417. VBIBarBac6912_0552.
TIGR	BARBAKC583_0571.
Phylogenomic databases
eggNOG	COG0085.
HOGENOM	HBG285547.
OMA	QRDLTYA.
ProtClustDB	PRK00405.
Enzyme and pathway databases
BioCyc	BBAC360095:BARBAKC583_0571-MONOMER.
Family and domain databases
HAMAP	MF_01321. RNApol_bact_RpoB. [Tree]
InterPro	IPR010243. DNA-dir_RNA_pol_bsu_bac-type. IPR019462. DNA-dir_RNA_pol_bsu_external_1. IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
Gene3D	G3DSA:2.30.150.10. G3DSA:2.30.150.10. 1 hit. G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits. G3DSA:2.40.50.150. Ribosomal_L2. 2 hits.
KO	K03043.
PANTHER	PTHR20856:SF3. PTHR20856:SF3. 1 hit. PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 2 hits. PF04565. RNA_pol_Rpb2_3. 1 hit. PF10385. RNA_pol_Rpb2_45. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR02013. RpoB. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB_BARBK

Accession

Primary (citable) accession number: A1USC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program